ADAMTSL4 Polyclonal antibody

ADAMTSL4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15304-1-AP

Synonyms

ADAMTS like 4, ADAMTS like protein 4, ADAMTSL 4, ADAMTSL4, TSRC1, UNQ2803/PRO34012

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Validation Data Gallery

• 

  WB analysis of mouse brain using 15304-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15304-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15304-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15304-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15304-1-AP

  IP result of anti-ADAMTSL4 (IP:15304-1-AP, 4ug; Detection:15304-1-AP 1:500) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of mouse skeletal muscle using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15304-1-AP (ADAMTSL4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15304-1-AP (ADAMTSL4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15304-1-AP (ADAMTSL4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15304-1-AP (ADAMTSL4 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human liver tissue, mouse lung tissue, mouse skeletal muscle tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse skeletal muscle tissue, human colon cancer tissue, human liver tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

15304-1-AP targets ADAMTSL4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAMTSL4 fusion protein Ag7781
• Full Name: ADAMTS-like 4
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC027478
• Gene Symbol: ADAMTSL4
• Gene ID (NCBI): 54507
• RRID: AB_2222624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IP,IF	Matrix Biol; Interactions between lysyl oxidases and ADAMTS proteins suggest a novel crosstalk between two extracellular matrix families.; Authors - Rohtem Aviram
human	IHC, WB	Front Mol Biosci; An Extracellular Matrix-Based Signature Associated With Immune Microenvironment Predicts the Prognosis and Therapeutic Responses of Patients With Oesophageal Squamous Cell Carcinoma.; Authors - Hongpan Zhang
human	WB	Oncotarget; Correlation of five secretory proteins with the nasopharyngeal carcinoma metastasis and the clinical applications.; Authors - Ya Tao
rat	IHC	Int J Nanomedicine; Pioglitazone-Loaded Cartilage-Targeted Nanomicelles (Pio@C-HA-DOs) for Osteoarthritis Treatment; Authors - Junyan Chen

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