ADAP1 Recombinant antibody

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  WB analysis using 84443-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84443-1-RR (ADAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 84443-1-RR

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 84443-1-RR (ADAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 84443-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84443-1-RR (ADAP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84443-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84443-1-RR (ADAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ADAP1 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 84443-1-RR

Clone No.241370D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

241370D6, Arf-GAP with dual PH domain-containing protein 1, ArfGAP with dual PH domains 1, CENTA1, Centaurin alpha 1

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293T cells, mouse small intestine tissue, rat kidney tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84443-1-RR targets ADAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ADAP1 fusion protein Ag4898
• Full Name: ArfGAP with dual PH domains 1
• Calculated Molecular Weight: 374 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC033747
• Gene Symbol: ADAP1
• Gene ID (NCBI): 11033
• RRID: AB_3671968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O75689
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.