ADAR1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 85009-1-RR (same clone as 85009-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85009-1-RR (ADAR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85009-1-PBS in a different storage buffer formulation.

ADAR1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 85009-1-PBS

Clone No.241595E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

ADAR, DRADA, Double-stranded RNA-specific adenosine deaminase, adenosine deaminase RNA specific, 241595E8

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Product Information

85009-1-PBS targets ADAR1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5609

  Product name: Recombinant human ADAR1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 877-1226 aa of BC038227

  Sequence: DSEDMGVVVSLGTGNRCVKGDSLSLKGETVNDCHAEIISRRGFIRFLYSELMKYNSQTAKDSIFEPAKGGEKLQIKKTVSFHLYISTAPCGDGALFDKSCSDRAMESTESRHYPVFENPKQGKLRTKVENGEGTIPVESSDIVPTWDGIRLGERLRTMSCSDKILRWNVLGLQGALLTHFLQPIYLKSVTLGYLFSQGHLTRAICCRVTRDGSAFEDGLRHPFIVNHPKVGRVSIYDSKRQSGKTKETSVNWCLADGYDLEILDGTRGTVDGPRNELSRVSKKNIFLLFKKLCSFRYRRDLLRLSYGEAKKAARDYETAKNYFKKGLKDMGYGNWISKPQEEKNFYLCPV

• Full Name: adenosine deaminase, RNA-specific
• Calculated Molecular Weight: 136 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC038227
• Gene Symbol: ADAR1
• Gene ID (NCBI): 103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P55265
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region(PMID:15556947). The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an IFN inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters(PMID:11111054). It has 5 isoforms produced by alternative promoter usage and alternative splicing. Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH).ADAR1 can form respective homodimers, and this association is essential for its enzymatic activities(PMID:17428802).