ADCK5 Polyclonal antibody

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  WB analysis of mouse brain using 17579-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17579-1-AP (ADCK5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17579-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17579-1-AP (ADCK5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 17579-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17579-1-AP (ADCK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ADCK5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17579-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

EC:2.7.11.-, Uncharacterized aarF domain-containing protein kinase 5

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17579-1-AP targets ADCK5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11094

  Product name: Recombinant human ADCK5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-169 aa of BC031570

  Sequence: MRAHAAALGVQDYLLFAEMLMQRPVRLGQLWGSHLLSREEAAYMVDMARERFEAVMAVLRELPRPMLLVLRNINTVRAINVALGAPVDRYFLMAKRAVRGWSRLAGATYRGVYGTSLLRHAKVVWEMLKFEVALRLETLAMRLTALLARALVHLSLVPPAEELYQYLET

• Full Name: aarF domain containing kinase 5
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC031570
• Gene Symbol: ADCK5
• Gene ID (NCBI): 203054
• RRID: AB_2242313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q3MIX3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADCK5 (AarF Domain Containing Kinase 5) is a protein kinase belonging to the ADCK protein family. Named for its AarF domain, this family plays important roles in cellular energy metabolism and mitochondrial function regulation. ADCK5 is primarily expressed in human tissues and participates in the biosynthesis of Coenzyme Q (CoQ). CoQ is a critical component of the mitochondrial electron transport chain, essential for cellular energy production. ADCK5 contains ubiquitination modification sites, and the experimentally detected molecular weight may range between 65-75 kDa.