Tested Applications
| Positive WB detected in | SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55067-1-AP targets ADCY1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | adenylate cyclase 1 (brain) |
| Calculated Molecular Weight | 123 kDa |
| Observed Molecular Weight | 123 kDa |
| GenBank Accession Number | NM_021116 |
| Gene Symbol | ADCY1 |
| Gene ID (NCBI) | 107 |
| RRID | AB_10858391 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08828 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ADCY1 belongs to the adenylyl cyclase class-4/guanylyl cyclase family. It is a calmodulin-sensitive adenylyl cyclase. ADCY1 may be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning. The antibody is specific to ADCY1.
Publications
What published studies show
ADCY1 Polyclonal antibody (Cat# 55067-1-AP) has a total of 4 citations appearing in Proceedings of the National Academy of Sciences, Diabetes, Frontiers in Pharmacology, and Frontiers in Cell and Developmental Biology. The associated research fields include neuroscience (striatal cAMP signaling and hippocampal synaptic remodeling), metabolism and diabetes (glucose intolerance via pancreatic β-cell insulin secretion), and cardiovascular pharmacology (sino-atrial node beat rate regulation).
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A KCTD1 regulation of Adenylyl cyclase type 5 adjusts striatal cAMP signaling | ||
Diabetes Visceral adipocyte-derived extracellular vesicle miR-27a-5p elicits glucose intolerance by inhibiting pancreatic β-cell insulin secretion | ||
Front Pharmacol Inhibition of adenylyl cyclase 1 by ST034307 inhibits IP3-evoked changes in sino-atrial node beat rate | ||
Front Cell Dev Biol HIIT-induced lactate/GPR81 signaling with dual branches converging on ERK1/2 contributes to hippocampal synaptic remodeling and memory improvement. |
