ADCY3 Polyclonal antibody

ADCY3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19492-1-AP

Synonyms

AC III, AC3, ADCY3, adenylate cyclase 3, Adenylate cyclase type 3, Adenylate cyclase type III, Adenylyl cyclase 3, ATP pyrophosphate lyase 3, KIAA0511



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human brain tissue
Positive IP detected inMDCK cells
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells,

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19492-1-AP targets ADCY3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name adenylate cyclase 3
Calculated molecular weight 129 kDa
Observed molecular weight 170-180 kDa
GenBank accession numberNM_004036
Gene symbol ADCY3
Gene ID (NCBI) 109
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADCY3 is an adenylate cyclase (AC) functioning to convert ATP to cAMP in response to signals initiated by activation of Gs-coupled receptors. To date, 10 isoforms of AC have been cloned in mammals. Initially identified in olfactory cilia, ADCY3 is regarded as the olfactory isoform and required for detection of odorants. Recently it has been found that outside of the olfactory epithelium the olfactory-like signaling pathway including AC3 may also have a key role in spermatogenesis and spermatozoa functions. In addition, the ciliary location of AC3 makes it as a prominent marker for primary cilia in brain.AC3 is typically found at 130 kDa in olfactory tissues.In nonolfactory tissues (such as sperm), this protein is reported to migrate with a molecular mass of 55 kDa,occasionally accompanied by additional bands at either 90 or 130 kDa(PMID:9539154).

Protocols

Product Specific Protocols
WB protocol for ADCY3 antibody 19492-1-APDownload protocol
IHC protocol for ADCY3 antibody 19492-1-APDownload protocol
IF protocol for ADCY3 antibody 19492-1-APDownload protocol
IP protocol for ADCY3 antibody 19492-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Rep

Phenotypic Screen with TSC-Deficient Neurons Reveals Heat-Shock Machinery as a Druggable Pathway for mTORC1 and Reduced Cilia.

Authors - Alessia Di Nardo
mouseIHC

Acta Physiol (Oxf)

Aromatherapy: Activating Olfactory Calcium-sensing Receptors Impairs Renal Hemodynamics via Sympathetic Nerve-mediated Vasoconstriction.

Authors - Shih-Pin Lee