ADCY5 Polyclonal antibody

ADCY5 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30153-1-AP

Synonyms

AC5, ADCY5, adenylate cyclase 5, Adenylate cyclase type 5, Adenylate cyclase type V, Adenylyl cyclase 5, ATP pyrophosphate lyase 5

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Validation Data Gallery

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  IHC staining of rat brain using 30153-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30153-1-AP (ADCY5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 30153-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30153-1-AP (ADCY5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 30153-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ADCY5 antibody (30153-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 30153-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ADCY5 antibody (30153-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30153-1-AP targets ADCY5 in IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADCY5 fusion protein Ag32812
• Full Name: adenylate cyclase 5
• Calculated Molecular Weight: 139 kDa
• Observed Molecular Weight: 139 kDa
• GenBank Accession Number: NM_183357
• Gene Symbol: ADCY5
• Gene ID (NCBI): 111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADCY5 belongs to the adenylate cyclase (ADCY) superfamily which is a group of glycoproteins regulating intracellular signaling (PMID: 35832555). ADCY5 is expressed only in neurons and all neurons express ADCY5 (PMID: 31970214). Gain-of-function mutations in ADCY5 is linked to a rare movement-onset disorder called ADCY5-related dyskinesia (PMID: 32627162).