ADCY6 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• ADCY6 Polyclonal antibody (14616-1-AP)

• KD/KO Validated

ADCY6 Polyclonal Antibody for WB, IP, ELISA

Cat No. 14616-1-AP

Publications(8)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, CoIP, ELISA

Synonyms

AC6, ATP pyrophosphate-lyase 6, Ca(2+)-inhibitable adenylyl cyclase, EC:4.6.1.1

• 

  WB analysis of human brain using 14616-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14616-1-AP (ADCY6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of SKOV-3 using 14616-1-AP

  WB result of ADCY6 antibody (14616-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADCY6 transfected SKOV-3 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 14616-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14616-1-AP (ADCY6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 14616-1-AP

  IP result of anti-ADCY6 (IP:14616-1-AP, 4ug; Detection:14616-1-AP 1:300) with mouse brain tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive WB detected in: human brain tissue, mouse brain tissue, SKOV-3 cells
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14616-1-AP targets ADCY6 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6218

  Product name: Recombinant human ADCY6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 310-655 aa of BC064923

  Sequence: AEVSQRQAFQETRGYIQARLHLQHENRQQERLLLSVLPQHVAMEMKEDINTKKEDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQELVMTLNELFARFDKLAAENHCLRIKILGDCYYCVSGLPEARADHAHCCVEMGVDMIEAISLVREVTGVNVNMRVGIHSGRVHCGVLGLRKWQFDVWSNDVTLANHMEAGGRAGRIHITRATLQYLNGDYEVEPGRGGERNAYLKEQHIETFLILGASQKRKEEKAMLAKLQRTRANSMEGLMPRWVPDRAFSRTKDSKAFRQMGIDDSSKDNRGTQDALNPEDEVDEFLSRAIDARSIDQLRKDHVRRFLLTFQRE

• Full Name: adenylate cyclase 6
• Calculated Molecular Weight: 131 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: BC064923
• Gene Symbol: ADCY6
• Gene ID (NCBI): 112
• RRID: AB_2242343
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43306
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adenylate cyclase 6 (ADCY6) belongs to the membrane-bound adenylate cyclase family. Increasing evidence has proven that ADCY6 is involved in pathophysiological processes of the body, such as the stability of cardiovascular system function, glucose and lipid metabolism, and even carcinogenesis.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	CoIP	Nat Commun; Multiple cAMP/PKA complexes at the STIM1 ER/PM junction specified by E-Syt1 and E-Syt2 reciprocally gates ANO1 (TMEM16A) via Ca2; Authors - Wei-Yin Lin
mouse	WB	Nat Commun; Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH); Authors - Xinzhi Li
mouse	WB	J Cell Biol; Sonic Hedgehog activates prostaglandin signaling to stabilize primary cilium length; Authors - Shariq S Ansari
mouse	WB	Mol Metab; Purβ promotes hepatic glucose production by increasing Adcy6 transcription.; Authors - Linna Jia
human	WB	Mol Pharmacol; Activity of adenylyl cyclase type 6 is suppressed by direct binding of the cytoskeletal protein 4.1G.; Authors - Masaki Saito
human	WB	Pathol Oncol Res; Knockdown of GPSM1 Inhibits the Proliferation and Promotes the Apoptosis of B-Cell Acute Lymphoblastic Leukemia Cells by Suppressing the ADCY6-RAPGEF3-JNK Signaling Pathway.; Authors - Ye Zhang

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

Charlotte (Verified Customer) (07-27-2022)

1 and 2 : WT starved and ShhN stimulated. 3 to 6 are ADC6 KO cells with 2 different gRNAs. 3/4 is one gRNA and 5/6 is another one. 3 and 5 are starved while 4 and 6 are ShhN stimulated

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: NIH-3T3

ADCY6 Antibody Western Blot validation (1/500 dilution) in NIH-3T3 (Cat no:14616-1-AP)