ADCY8 Polyclonal antibody

• Featured Product
• KD/KO Validated

ADCY8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 55065-1-AP

Synonyms

AC8, ADCY3, ADCY8, adenylate cyclase 8 (brain), Adenylate cyclase type 8, Adenylate cyclase type VIII, Adenylyl cyclase 8, ATP pyrophosphate lyase 8, HBAC1

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Validation Data Gallery

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  WB analysis of Neuro-2a using 55065-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 55065-1-AP (ADCY8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 55065-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 55065-1-AP (ADCY8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 55065-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55065-1-AP (ADCY8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Neuro-2a cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Product Information

55065-1-AP targets ADCY8 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: adenylate cyclase 8 (brain)
• Calculated Molecular Weight: 140 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: NM_001115
• Gene Symbol: ADCY8
• Gene ID (NCBI): 114
• RRID: AB_10859258
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADCY8 Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. is a membrane-bound, calcium-stimulable adenylyl cyclase. May be involved in learning, in memory and in drug dependence. The antibody is specific to ADCY8.

Publications

Species	Application	Title
human	IF	Phytochemistry; Hypoglycemic flavonoids from Selaginella tamariscina (P.Beauv.) Spring.; Authors - Hong-Ping Long
human	WB	Function (Oxf); AKAP79 Orchestrates a Cyclic AMP Signalosome Adjacent to Orai1 Ca2+ Channels.; Authors - Pulak Kar
human	WB	Nat Commun; A calcium/cAMP signaling loop at the ORAI1 mouth drives channel inactivation to shape NFAT induction.; Authors - Xuexin Zhang ; KD Validated
rat	WB,IF	Mol Ther; Inhibition of adenylyl cyclase 8 prevents the upregulation of Orai1 channel which improves cardiac function after myocardial infarction; Authors - Débora Falcón