ADH1B Polyclonal antibody

ADH1B Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17165-1-AP

Synonyms

ADH1B, ADH2, Alcohol dehydrogenase 1B



Tested Applications

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17165-1-AP targets ADH1B in WB, IP, IHC, IF, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADH1B fusion protein Ag10630
Full Name alcohol dehydrogenase 1B (class I), beta polypeptide
Calculated Molecular Weight 375 aa, 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC033009
Gene Symbol ADH1B
Gene ID (NCBI) 125
RRIDAB_2878353
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).

Protocols

Product Specific Protocols
WB protocol for ADH1B antibody 17165-1-APDownload protocol
IP protocol for ADH1B antibody 17165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Cell Death Discov

Senescence as a dictator of patient outcomes and therapeutic efficacies in human gastric cancer.

Authors - Lulin Zhou
IF

Endocrinology

De novo-synthesized retinoic acid in ovarian antral follicles enhances FSH-mediated ovarian follicular cell differentiation and female fertility.

Authors - Tomoko Kawai
ratWB

Arch Biochem Biophys

Baicalin ameliorates alcohol-induced hepatic steatosis by suppressing SREBP1c elicited PNPLA3 competitive binding to ATGL.

Authors - Pan Li
IHC

Exp Eye Res

Proteomic analysis reveals the heterogeneity of metabolic reprogramming in lacrimal gland tumors.

Authors - Jie Yang
ratWB

J Sci Food Agric

Probiotic-fermented Pueraria lobata (Willd.) Ohwi alleviates alcoholic liver injury by enhancing antioxidant defense and modulating gut microbiota

Authors - Wenjun Zhao
WB

Front Pharmacol

Identification of afatinib-associated ADH1B and potential small-molecule drugs targeting ADH1B for hepatocellular carcinoma

Authors - Yongxu Zhou