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ADH4 Polyclonal antibody

ADH4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 16474-1-AP


ADH 2, ADH4, Alcohol dehydrogenase 4

Tested Applications

Positive WB detected inHepG2 cells, mouse brain tissue, HeLa cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

16474-1-AP targets ADH4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADH4 fusion protein Ag9568
Full Name alcohol dehydrogenase 4 (class II), pi polypeptide
Calculated Molecular Weight 380 aa, 40 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC022319
Gene Symbol ADH4
Gene ID (NCBI) 127
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes the class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. ADH4 plays a critical role in alcoholism development and alcohol toxicology and is also associated with the development of esophageal cancer(PMID:33556337, 26039424, 24051444, 22147505).


Product Specific Protocols
WB protocol for ADH4 antibody 16474-1-APDownload protocol
IHC protocol for ADH4 antibody 16474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Immunol

Identification and validation of a tyrosine metabolism-related prognostic prediction model and characterization of the tumor microenvironment infiltration in hepatocellular carcinoma

Authors - Yangying Zhou

Front Oncol

Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.

Authors - Tingbo Ye