ADH4 Polyclonal antibody

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ADH4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16474-1-AP Copied!

Publications (9)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ADH-2, All-trans-retinol dehydrogenase [NAD(+)] ADH4, EC:1.1.1.105

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  WB analysis of HepG2 using 16474-1-AP

  WB result of ADH4 antibody (16474-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADH4 transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 16474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16474-1-AP (ADH4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 16474-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16474-1-AP (ADH4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 16474-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16474-1-AP (ADH4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human kidney using 16474-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16474-1-AP (ADH4 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 16474-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16474-1-AP (ADH4 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 16474-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16474-1-AP (ADH4 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 16474-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16474-1-AP (ADH4 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse brain tissue, HeLa cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16474-1-AP targets ADH4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9568

  Product name: Recombinant human ADH4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-380 aa of BC022319

  Sequence: MGTKGKVIKCKAAIAWEAGKPLCIEEVEVAPPKAHEVRIQIIATSLCHTDATVIDSKFEGLAFPVIVGHEAAGIVESIGPGVTNVKPGDKVIPLYAPLCRKCKFCLSPLTNLCGKISNLKSPASDQQLMEDKTSRFTCKGKPVYHFFGTSTFSQYTVVSDINLAKIDDDANLERVCLLGCGFSTGYGAAINNAKVTPGSTCAVFGLGGVGLSAVMGCKAAGASRIIGIDINSEKFVKAKALGATDCLNPRDLHKPIQEVIIELTKGGVDFALDCAGGSETMKAALDCTTAGWGSCTFIGVAAGSKGLTVFPEELIIGRTINGTFFGGWKSVDSIPKLVTDYKNKKFNLDALVTHTLPFDKISEAFDLMNQGKSIRTILIF

• Full Name: alcohol dehydrogenase 4 (class II), pi polypeptide
• Calculated Molecular Weight: 380 aa, 40 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC022319
• Gene Symbol: ADH4
• Gene ID (NCBI): 127
• RRID: AB_2223855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08319
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes the class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. ADH4 plays a critical role in alcoholism development and alcohol toxicology and is also associated with the development of esophageal cancer(PMID:33556337, 26039424, 24051444, 22147505).

Publications

What published studies show

The anti-ADH4 antibody (Cat# 16474-1-AP) has 9 citations published in journals including Cell Communications & Signaling, Cancer Cell International, Frontiers in Immunology, iScience, BMC Cancer, Drug Metabolism and Disposition, Frontiers in Oncology, and BMC Gastroenterology. Research fields span hepatocellular carcinoma prognosis, ferroptosis regulation, tumor microenvironment characterization, non-apoptotic cell death crosstalk, and drug metabolism involving alcohol dehydrogenase 4.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IHC	Cell Commun Signal; In vivo CRISPR screening identifies POU3F3 as a novel regulator of ferroptosis resistance in hepatocellular carcinoma via retinoic acid signaling; Authors - Yu Tian
human	IHC	Front Immunol; Identification and validation of a tyrosine metabolism-related prognostic prediction model and characterization of the tumor microenvironment infiltration in hepatocellular carcinoma; Authors - Yangying Zhou
human	WB,IHC	Cancer Cell Int; ADH4 deficiency activates the NRF2-CSF1 axis by increased lipid peroxidation in hepatocellular carcinoma.; Authors - Ping Yan
Human		iScience; Crosstalk of non-apoptotic RCD panel in hepatocellular carcinoma reveals the prognostic and therapeutic optimization; Authors - Shuo Li
human	IHC	BMC Cancer; ADH4-a potential prognostic marker for hepatocellular carcinoma with possible immune-related implications; Authors - Ling Li
human	WB	Drug Metab Dispos; Characterization of human alcohol dehydrogenase 4 and aldehyde dehydrogenase 2 as enzymes involved in the formation of 5-carboxylpirfenidone, a major metabolite of pirfenidone; Authors - Rei Sato