ADH4 Polyclonal antibody

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  WB analysis of HepG2 using 16474-1-AP

  WB result of ADH4 antibody (16474-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADH4 transfected HepG2 cells.

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  WB analysis using 16474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16474-1-AP (ADH4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16474-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16474-1-AP (ADH4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16474-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16474-1-AP (ADH4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16474-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16474-1-AP (ADH4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 16474-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16474-1-AP (ADH4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 16474-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16474-1-AP (ADH4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16474-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16474-1-AP (ADH4 antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

ADH4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16474-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ADH 2, ADH4, Alcohol dehydrogenase 4

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse brain tissue, HeLa cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 3 publications below

Product Information

16474-1-AP targets ADH4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADH4 fusion protein Ag9568
• Full Name: alcohol dehydrogenase 4 (class II), pi polypeptide
• Calculated Molecular Weight: 380 aa, 40 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC022319
• Gene Symbol: ADH4
• Gene ID (NCBI): 127
• RRID: AB_2223855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08319
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes the class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. ADH4 plays a critical role in alcoholism development and alcohol toxicology and is also associated with the development of esophageal cancer(PMID:33556337, 26039424, 24051444, 22147505).

Publications

Species	Application	Title
human	IHC	Front Immunol; Identification and validation of a tyrosine metabolism-related prognostic prediction model and characterization of the tumor microenvironment infiltration in hepatocellular carcinoma; Authors - Yangying Zhou
human	IHC	Front Oncol; Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.; Authors - Tingbo Ye
human	WB	Drug Metab Dispos; Characterization of human alcohol dehydrogenase 4 and aldehyde dehydrogenase 2 as enzymes involved in the formation of 5-carboxylpirfenidone, a major metabolite of pirfenidone; Authors - Rei Sato
human	IHC	BMC Cancer; ADH4-a potential prognostic marker for hepatocellular carcinoma with possible immune-related implications; Authors - Ling Li