ADH6 Polyclonal antibody

ADH6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13288-2-AP

Synonyms

EC:1.1.1.1, ADH-5

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Validation Data Gallery

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  WB analysis using 13288-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13288-2-AP (ADH6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13288-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13288-2-AP (ADH6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 13288-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13288-2-AP (ADH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 13288-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13288-2-AP (ADH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 13288-2-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13288-2-AP (ADH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 13288-2-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 13288-2-AP (ADH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 13288-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13288-2-AP (ADH6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 13288-2-AP

  Immunofluorescent analysis of MCF-7 cells, using ADH6 antibody 13288-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse liver tissue, human liver tissue, rat liver tissue
• Positive IHC detected in: human liver tissue, mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13288-2-AP targets ADH6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADH6 fusion protein Ag3984
• Full Name: alcohol dehydrogenase 6 (class V)
• Calculated Molecular Weight: 368 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC039065
• Gene Symbol: ADH6
• Gene ID (NCBI): 130
• RRID: AB_2273710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.