ADH6 Monoclonal antibody

ADH6 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F5C6

Cat no : 67709-1-Ig

Synonyms

ADH 5, ADH6, Alcohol dehydrogenase 6

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Validation Data Gallery

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  WB analysis of mouse liver using 67709-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67709-1-Ig (ADH6 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 67709-1-Ig

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 67709-1-Ig (ADH6 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 67709-1-Ig

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 67709-1-Ig (ADH6 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit kidney using 67709-1-Ig

  rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 67709-1-Ig (ADH6 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse kidney using 67709-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ADH6 antibody (67709-1-Ig, Clone: 1F5C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat kidney tissue, mouse kidney tissue, rabbit kidney tissue
• Positive IF detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67709-1-Ig targets ADH6 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

• Tested Reactivity: Human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ADH6 fusion protein Ag28845
• Full Name: alcohol dehydrogenase 6 (class V)
• Calculated Molecular Weight: 368 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC039065
• Gene Symbol: ADH6
• Gene ID (NCBI): 130
• RRID: AB_2918498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.