ADH6 Monoclonal antibody

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  WB analysis of mouse liver using 67709-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67709-1-Ig (ADH6 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 67709-1-Ig

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 67709-1-Ig (ADH6 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 67709-1-Ig

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 67709-1-Ig (ADH6 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit kidney using 67709-1-Ig

  rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 67709-1-Ig (ADH6 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse kidney using 67709-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ADH6 antibody (67709-1-Ig, Clone: 1F5C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

ADH6 Monoclonal Antibody for WB, IF-P, ELISA

Cat No. 67709-1-Ig

Clone No.1F5C6

Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit

Applications

WB, IF-P, ELISA

Synonyms

ADH 5, ADH6, Alcohol dehydrogenase 6

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat kidney tissue, mouse kidney tissue, rabbit kidney tissue
• Positive IF-P detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67709-1-Ig targets ADH6 in WB, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

• Tested Reactivity: Human, mouse, rat, rabbit
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28845

  Product name: Recombinant human ADH6 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-295 aa of BC039065

  Sequence: MSTTGQVIRCKAAILWKPGAPFSIEEVEVAPPKAKEVRIKVVATGLCGTEMKVLGSKHLDLLYPTILGHEGAGIVESIGEGVSTVKPGDKVITLFLPQCGECTSCLNSEGNFCIQFKQSKTQLMSDGTSRFTCKGKSIYHFGNTSTFCEYTVIKEISVAKIDAVAPLEKVCLISCGFSTGFGAAINTAKVTPGSTCAVFGLGGVGLSVVMGCKAAGAARIIGVDVNKEKFKKAQELGATECLNPQDLKKPIQEVLFDMTDAGIDFCFEAIGNLDVLAGRADSTSLNWLLIIWQRS

• Full Name: alcohol dehydrogenase 6 (class V)
• Calculated Molecular Weight: 368 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC039065
• Gene Symbol: ADH6
• Gene ID (NCBI): 130
• RRID: AB_2918498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P28332
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Mol Nutr Food Res; Protective Effect and Mechanism of L-Theanine on Acute Alcoholic Liver Injury in Mice; Authors - Meng-Yuan Liu