Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, PC-3 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15477-1-AP targets ADK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7783 Product name: Recombinant human ADK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-345 aa of BC003568 Sequence: MTSVRENILFGMGNPLLDISAVVDKDFLDKYSLKPNDQILAEDKHKELFDELVKKFKVEYHAGGSTQNSIKVAQWMIQQPHKAATFFGCIGIDKFGEILKRKAAEAHVDAHYYEQNEQPTGTCAACITGDNRSLIANLAAANCYKKEKHLDLEKNWMLVEKARVCYIAGFFLTVSPESVLKVAHHASENNRIFTLNLSAPFFSQFYKESLMKVMPYVDILFGNETEAATFAREQGFETKDIKEIAKKTQALPKMNSKRQRIVIFTQGRDDTIMATESEVTAFAVLDQDQKEIIDTNGAGDAFVGGFLSQLVSDKPLTECIRAGHYAASIIIRRTGCTFPEKPDFH Predict reactive species |
| Full Name | adenosine kinase |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 40-46 kDa |
| GenBank Accession Number | BC003568 |
| Gene Symbol | ADK |
| Gene ID (NCBI) | 132 |
| RRID | AB_2222085 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55263 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ADK antibody 15477-1-AP | Download protocol |
| WB protocol for ADK antibody 15477-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ADK Polyclonal antibody (Cat# 15477-1-AP) has 2 total citations. These appear in Neurochemical Research and Cardiovascular Drugs and Therapy. The cited research spans neurochemistry (NLRP3 inflammasome activation and epilepsy in mice) and cardiovascular pharmacology (SGLT2 inhibitors against doxorubicin-induced cardiotoxicity via adenosine kinase-mediated oxidative stress). Both studies used the antibody for Western blot in mouse models.
| Species | Application | Title |
|---|---|---|
Neurochem Res NLRP3 Inflammasome Activation Enhances ADK Expression to Accelerate Epilepsy in Mice | ||
Cardiovasc Drugs Ther Class Effect of SGLT2 Inhibitors Against Doxorubicin-Induced Cardiotoxicity via Regulating Adenosine Kinase Mediated-Cardiac Oxidative Stress.
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