ADO Polyclonal antibody

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  WB analysis of human brain using 16479-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16479-1-AP (ADO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 16479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16479-1-AP (ADO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 16479-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16479-1-AP (ADO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 16479-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16479-1-AP (ADO antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16479-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16479-1-AP (ADO antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

ADO Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16479-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

2 aminoethanethiol dioxygenase, 2-aminoethanethiol dioxygenase, C10orf22, Cysteamine dioxygenase, EC:1.13.11.19

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Tested Applications

• Positive WB detected in: fetal human brain tissue, human brain tissue, mouse spleen tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below

Product Information

16479-1-AP targets ADO in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9598

  Product name: Recombinant human ADO protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-270 aa of BC018660

  Sequence: MPRDNMASLIQRIARQACLTFRGSWGGRGASDRDAASGAEAPMQPGFPENLSKLKSLLTQLRAEDLNIAPRKATLQPLPPNLPPVTYMHIYETDGFSLGVFLLKSGTSIPLHDHPGMHGMLKVLYGTVRISCMDKLDAGGGQRPRALPPEQQFEPPLQPREREAVRPGVLRSRAEYTEASGPCILTPHRDNLHQIDAVEGPAAFLDILAPPYDPDDGRDCHYYRVLEPVRPKEASSSACDLPREVWLLETPQADDFWCEGEPYPGPKVFP

• Full Name: 2-aminoethanethiol (cysteamine) dioxygenase
• Calculated Molecular Weight: 270 aa, 30 kDa
• Observed Molecular Weight: 28 kDa-30 kDa
• GenBank Accession Number: BC018660
• Gene Symbol: ADO
• Gene ID (NCBI): 84890
• RRID: AB_2222204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96SZ5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADO, or 2-aminoethanethiol dioxygenase, cysteamine dioxygenase, is a thiol dioxygenase in humans that adds two oxygen atoms to cysteamine, forming hypotaurine (PMID: 39637922). ADO has been reported to exhibit two distinct biological functions with a nonheme iron center. It catalyzes oxidation of both cysteamine in sulfur metabolism and N-terminal cysteine-containing proteins or peptides, such as regulator of G protein signaling 5 (RGS5) (PMID: 34508780). ADO is ubiquitously expressed with the highest levels in brain, heart, and skeletal muscle. Western blot analysis detected two bands at 28~30 kDa as indicated in publications (PMID: 17581819).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Biomol Ther (Seoul); Taurine Synthesis by 2-Aminoethanethiol Dioxygenase as a Vulnerable Metabolic Alteration in Pancreatic Cancer; Authors - Hoonsik Nam ; KD Validated
human	WB	Cell Death Discov; ADO/hypotaurine: a novel metabolic pathway contributing to glioblastoma development.; Authors - Dachuan Shen ; KO Validated
		Free Radic Biol Med; Taurine ameliorates radiation-induced oxidative stress in bone marrow mesenchymal stromal cells and promotes osteogenesis; Authors - Heng Chen