ADPRH Polyclonal antibody

ADPRH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17911-1-AP

Synonyms

ADP ribosylarginine hydrolase, ADPRH, ARH1

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Validation Data Gallery

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  WB analysis using 17911-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17911-1-AP (ADPRH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17911-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17911-1-AP (ADPRH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 17911-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17911-1-AP (ADPRH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17911-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17911-1-AP (ADPRH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 17911-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17911-1-AP (ADPRH antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17911-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17911-1-AP (ADPRH antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

The immunogen of 17911-1-AP is ADPRH Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADPRH fusion protein Ag12311
• Full Name: ADP-ribosylarginine hydrolase
• Calculated Molecular Weight: 357 aa, 40 kDa
• Observed Molecular Weight: 36-39 kDa
• GenBank Accession Number: BC063883
• Gene Symbol: ADPRH
• Gene ID (NCBI): 141
• RRID: AB_2878466
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	J Cancer; ADPRH is a prognosis-related biomarker and correlates with immune infiltrates in low grade glioma.; Authors - Chunyu Zhang