ADRA2A Polyclonal antibody

ADRA2A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14266-1-AP

Synonyms

ADRA2, ADRA2A, ADRA2R, ADRAR, Alpha 2A adrenergic receptor, Alpha 2A adrenoceptor, Alpha 2A adrenoreceptor, Alpha 2AAR, ALPHA2AAR, ZNF32



Tested Applications

Positive WB detected inPC-12 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14266-1-AP targets ADRA2A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADRA2A fusion protein Ag5562
Full Name adrenergic, alpha-2A-, receptor
Calculated molecular weight 49 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC050414
Gene symbol ADRA2A
Gene ID (NCBI) 150
RRIDAB_2636822
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADRA2A (Adrenoceptor Alpha-2) belongs to the adrenoceptor alpha-2 family of GPCRs coupled to the inhibitory G-alpha subunit (Gi) that suppresses adenylyl cyclase activity, thereby suppressing the cyclization of AMP to cAMP (PMID: 38132444). ADRA2A inhibits the flow of sympathetic nerves in the central nervous system and plays a major role in sedation, anxiolysis, and antinociception (PMID: 33915198).

Protocols

Product Specific Protocols
WB protocol for ADRA2A antibody 14266-1-APDownload protocol
IHC protocol for ADRA2A antibody 14266-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Int J Mol Sci

Chronic Administrations of Guanfacine on Mesocortical Catecholaminergic and Thalamocortical Glutamatergic Transmissions.

Authors - Kouji Fukuyama
humanWB

Am J Cancer Res

SNHG15, a p53-regulated lncRNA, suppresses cisplatin-induced apoptosis and ROS accumulation through the miR-335-3p/ZNF32 axis.

Authors - Yue-Feng Sun
mouseIHC

Front Immunol

β2-Adrenoceptor Deficiency Results in Increased Calcified Cartilage Thickness and Subchondral Bone Remodeling in Murine Experimental Osteoarthritis.

Authors - Gundula Rösch
mouseWB

Front Mol Neurosci

PMID: 28955200

Authors - Yong-Jie Wang
mouseWB

Exp Neurol

Two-hour acute restraint stress facilitates escape behavior and learning outcomes through the activation of the Cdk5/GR P S211 pathway in male mice.

Authors - Bin Wang
ratWB

J Cell Mol Med

The α2AR/Caveolin-1/p38MAPK/NF-κB axis explains dexmedetomidine protection against lung injury following intestinal ischaemia-reperfusion.

Authors - Lin Xu