ADRB1 Polyclonal antibody

ADRB1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 28323-1-AP

Synonyms

ADRB1, ADRB1R, adrenergic, beta 1 , receptor, B1AR, Beta 1 adrenergic receptor, Beta 1 adrenoceptor, Beta 1 adrenoreceptor, BETA1AR, RHR

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Validation Data Gallery

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  WB analysis using 28323-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28323-1-AP (ADRB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

28323-1-AP targets ADRB1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADRB1 fusion protein Ag28223
• Full Name: adrenergic, beta-1-, receptor
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: NM_000684
• Gene Symbol: ADRB1
• Gene ID (NCBI): 153
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADRB1 (adrenergic receptor beta 1), a member of the G protein-coupled receptor (GPCR) superfamily, responds to catecholamine stimulation. In the heart, ADRB1 is the predominant subtype expressed and mediate increases in inotropy and chronotropy when stimulated. ADRB1 mediates a wide range of cardiovascular physiological responses and has been of great interest to researchers as a therapeutic target for cardiovascular diseases. Moreover, there are three subtypes of ADRBs (ADRB1, ADRB2 and ADRB3) encoded by three separate genes (PMID:16636683; 33372534; 33593484).

Publications

Species	Application	Title
human,mouse	WB	iScience; Region-specific sympatho-adrenergic regulation of specialized vasculature in bone homeostasis and regeneration; Authors - Hao-Kun Xu
human	WB	Oncol Lett; β‑adrenergic receptor activation promotes the proliferation of HepG2 cells via the ERK1/2/CREB pathways; Authors - Xingcheng Lin
rat	WB	Brain Res; The α1 adrenoceptor antagonist prazosin potentiates morphine induced conditioned place preference in rats; Authors - Wanyu Tu