ADRM1 Monoclonal antibody, PBS Only

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  WB analysis using 66464-1-Ig (same clone as 66464-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66464-1-Ig (ADRM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66464-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66464-1-Ig (same clone as 66464-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ADRM1 antibody (66464-1-Ig, Clone: 1A1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66464-1-PBS in a different storage buffer formulation.

ADRM1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 66464-1-PBS

Clone No.1A1A3

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

adhesion regulating molecule 1, ADRM1, ARM 1, ARM1, GP110, hRpn13, Rpn13, Rpn13 homolog

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Product Information

66464-1-PBS targets ADRM1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24699

  Product name: Recombinant human ADRM1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-407 aa of BC017245

  Sequence: MTTSGALFPSLVPGSRGASNKYLVEFRAGKMSLKGTTVTPDKRKGLVYIQQTDDSLIHFCWKDRTSGNVEDDLIIFPDDCEFKRVPQCPSGRVYVLKFKAGSKRLFFWMQEPKTDQDEEHCRKVNEYLNNPPMPGALGASGSSGHELSALGGEGGLQSLLGNMSHSQLMQLIGPAGLGGLGGLGALTGPGLASLLGSSGPPGSSSSSSSRSQSAAVTPSSTTSSTRATPAPSAPAAASATSPSPAPSSGNGASTAASPTQPIQLSDLQSILATMNVPAGPAGGQQVDLASVLTPEIMAPILANADVQERLLPYLPSGESLPQTADEIQNTLTSPQFQQALGMFSAALASGQLGPLMCQFGLPAEAVEAANKGDVEAFAKAMQNNAKPEQKEGDTKDKKDEEEDMSLD

• Full Name: adhesion regulating molecule 1
• Calculated Molecular Weight: 407 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC017245
• Gene Symbol: ADRM1
• Gene ID (NCBI): 11047
• RRID: AB_2881832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16186
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.