ADSS Polyclonal antibody

ADSS Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16373-1-AP

Synonyms

ADEH, adenylosuccinate synthase, ADSS, AdSS 2, ADSS2, AMPSase 2, IMP aspartate ligase 2

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Validation Data Gallery

• 

  WB analysis of mouse skeletal muscle using 16373-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16373-1-AP (ADSS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 16373-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16373-1-AP (ADSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 16373-1-AP

  IP result of anti-ADSS (IP:16373-1-AP, 4ug; Detection:16373-1-AP 1:300) with mouse testis tissue lysate 4000 ug.

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

16373-1-AP targets ADSS in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADSS fusion protein Ag9298
• Full Name: adenylosuccinate synthase
• Calculated Molecular Weight: 456 aa, 50 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC012356
• Gene Symbol: ADSS
• Gene ID (NCBI): 159
• RRID: AB_2225676
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.; Authors - Eunus S Ali
human	WB	Cell Rep; The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.; Authors - Gerta Hoxhaj
human	WB, IHC	PLoS Biol; Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.; Authors - Yajing Lv