AEG-1 Recombinant monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP01497-1

  Sandwich ELISA standard curve of MP01497-1, Human AEG-1/MTDH Recombinant Matched Antibody Pair - PBS only. 84686-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4840. 84686-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP01497-1

  The mean AEG-1/MTDH concentration was determined to be 77.3 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 84.5 ng/mL in HeLa cell extract based on a 1.3 mg/mL extract load, 80.3 ng/mL in HepG2 cell extract based on a 3.3 mg/mL extract load and 3.8 ng/mL in mouse liver tissue extract based on a 3.7 mg/mL extract load.

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  Cytometric bead array standard curve of MP01497-1

  Cytometric bead array standard curve of MP01497-1, AEG-1/MTDH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84686-1-PBS. Detection antibody: 84686-2-PBS. Standard: Ag4840. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84686-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84686-1-PBS against Human AEG-1 was performed. The affinity constant is below 1 pM.

AEG-1 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84686-1-PBS

Clone No.242110C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

MTDH, 3D3/LYRIC, AEG 1, AEG1, Astrocyte elevated gene-1 protein

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Product Information

84686-1-PBS targets AEG-1 as part of a matched antibody pair:

MP01497-1: 84686-1-PBS capture and 84686-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4840

  Product name: Recombinant human AEG-1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 233-582 aa of BC045642

  Sequence: EQLTTASFPVGSKKNKGDSHLNVQVSNFKSGKGDSTLQVSSGLNENLTVNGGGWNEKSVKLSSQISAGEEKWNSVSPASAGKRKAEPSAWSQDTGDANTNGKDWGRSWSDRSIFSGIGSTAEPVSQSTTSDYQWDVSRNQPYIDDEWSGLNGLSSADPNSDWNAPAEEWGNWVDEERASLLKSQEPIPDDQKVSDDDKEKGEGALPTGKSKKKKKKKKKQGEDNSTAQDTEELEKEIREDLPVNTSKTRPKQEKAFSLKTISTSDPAEVLVKNSQPIKTLPPATSTEPSVILSKSDSDKSSSQVPPILQETDKSKSNTKQNSVPPSQTKSETSWESPKQIKKKKKARRET

• Full Name: metadherin
• Calculated Molecular Weight: 582 aa, 64 kDa
• GenBank Accession Number: BC045642
• Gene Symbol: AEG-1/MTDH
• Gene ID (NCBI): 92140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q86UE4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.