AFF4 Polyclonal antibody

AFF4 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 13197-1-AP

Synonyms

AF4/FMR2 family member 4, AF4/FMR2 family, member 4, AF5Q31, AFF4, MCEF, Protein AF 5q31

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Validation Data Gallery

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  WB analysis of Jurkat using 13197-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13197-1-AP (AFF4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 13197-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13197-1-AP (AFF4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

Sample-dependent, check data in validation data gallery

Product Information

13197-1-AP targets AFF4 in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AFF4 fusion protein Ag3931
• Full Name: AF4/FMR2 family, member 4
• Calculated molecular weight: 365 aa, 40 kDa
• Observed molecular weight: 150 kDa
• GenBank accession number: BC025700
• Gene symbol: AFF4
• Gene ID (NCBI): 27125
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.