AFG3L2 Monoclonal antibody, PBS Only

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  WB analysis using 68517-1-Ig (same clone as 68517-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68517-1-Ig (AFG3L2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68517-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 68517-1-Ig (same clone as 68517-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AFG3L2 antibody (68517-1-Ig, Clone: 1C12F3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68517-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68517-1-Ig (same clone as 68517-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug AFG3L2 Monoclonal antibody (68517-1-Ig, Clone:1C12F3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 68517-1-PBS in a different storage buffer formulation.

AFG3L2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 68517-1-PBS

Clone No.1C12F3

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

1C12F3, AFG3 like protein 2, AFG3L 2, AFG3-like protein 2, EC:3.4.24.-

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Product Information

68517-1-PBS targets AFG3L2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7180

  Product name: Recombinant human AFG3L2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 449-797 aa of BC065016

  Sequence: ILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKVHLRPLKLDSTLEKDKLARKLASLTPGFSGADVANVCNEAALIAARHLSDSINQKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARLIDDEVRILINDAYKRTVALLTEKKADVEKVALLLLEKEVLDKNDMVELLGPRPFAEKSTYEEFVEGTGSLDEDTSLPEGLKDWNKEREKEKEEPPGEKVAN

• Full Name: AFG3 ATPase family gene 3-like 2 (yeast)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC065016
• Gene Symbol: AFG3L2
• Gene ID (NCBI): 10939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y4W6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537) and it can exsit as a truncated 65 kDa protein(PMID:18337413). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5).