AFM Polyclonal antibody
AFM Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 25280-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human blood tissue |
Positive IP detected in | human plasma tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
The immunogen of 25280-1-AP is AFM Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | AFM fusion protein Ag16866 |
Full Name | afamin |
Calculated Molecular Weight | 599 aa, 69 kDa |
Observed Molecular Weight | 70-85 kDa |
GenBank Accession Number | BC109020 |
Gene Symbol | AFM |
Gene ID (NCBI) | 173 |
RRID | AB_2880005 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.
Protocols
Product Specific Protocols | |
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WB protocol for AFM antibody 25280-1-AP | Download protocol |
IP protocol for AFM antibody 25280-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomed Chromatogr Revealing the mechanism of Gualou-Xiebai against myocardial ischemia based on network pharmacology and energy metabolism strategies |