AFP Monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP50072-1

  Sandwich ELISA standard curve of MP50072-1, AFP Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68686-1-PBS. Detection antibody: 68686-2-PBS. Standard: Eg0539. Range: 31.25-2000 pg/mL.

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  Sample test of MP50072-1

  Serum of sixteen individual healthy human donors was measured. The AFP concentration of detected samples was determined to be 2,982.2 pg/mL with a range of 1,552.8-5,209.8 pg/mL.

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  Sample test of MP50072-1

  Urine of sixteen individual healthy human donors was measured. The AFP concentration of detected samples was determined to be 2,894.0 ng/mL with a range of 433.9-5,520.6 pg/mL.

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  Sample test of MP50072-1

  HepG2 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human AFP, and measured 4,199.4 ng/mL.

AFP Monoclonal Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 68686-2-PBS

Clone No.1D11G5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Alpha fetoglobulin, alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein

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Product Information

68686-2-PBS targets AFP as part of a matched antibody pair:

MP50072-1: 68686-1-PBS capture and 68686-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg0539

  Product name: Recombinant Human AFP protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 19-609 aa of BC027881

  Sequence: RTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV

• Full Name: alpha-fetoprotein
• Calculated Molecular Weight: 69 kDa
• GenBank Accession Number: BC027881
• Gene Symbol: AFP
• Gene ID (NCBI): 174
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P02771
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.