AGA Polyclonal antibody, PBS Only

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• AGA Polyclonal antibody, PBS Only (17299-1-PBS)

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  IHC staining of mouse kidney using 17299-1-AP (same clone as 17299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17299-1-AP (AGA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 17299-1-AP (same clone as 17299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17299-1-AP (AGA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17299-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17299-1-AP (same clone as 17299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17299-1-AP (AGA Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 17299-1-AP (same clone as 17299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17299-1-AP (AGA Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17299-1-PBS in a different storage buffer formulation.

AGA Polyclonal Antibody for IHC, Indirect ELISA

Cat No. 17299-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, Indirect ELISA

Synonyms

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Product Information

17299-1-PBS targets AGA in IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10681

  Product name: Recombinant human AGA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-346 aa of BC012392

  Sequence: MARKSNLPVLLVPFLLCQALVRCSSPLPLVVNTWPFKNATEAAWRALASGGSALDAVESGCAMCEREQCDGSVGFGGSPDELGETTLDAMIMDGTTMDVGAVGDLRRIKNAIGVARKVLEHTTHTLLVGESATTFAQSMGFINEDLSTSASQALHSDWLARNCQPNYWRNVIPDPSKYCGPYKPPGILKQDIPIHKETEDDRGHDTIGMVVIHKTGHIAAGTSTNGIKFKIHGRVGDSPIPGAGAYADDTAGAAAATGNGDILMRFLPSYQAVEYMRRGEDPTIACQKVISRIQKHFPEFFGAVICANVTGSYGAACNKLSTFTQFSFMVYNSEKNQPTEEKVDCI

• Full Name: aspartylglucosaminidase
• Calculated Molecular Weight: 346 aa, 37 kDa
• GenBank Accession Number: BC012392
• Gene Symbol: AGA
• Gene ID (NCBI): 175
• RRID: AB_2878378
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20933
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AGA(Aspartylglucosaminidase) is also named as N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase and glycosylasparaginase.The open reading frame of the readthrough transcript would indicate AGA close to 40 kDa.It can be cleaved into 2 chains:the 24 kDa α- and 17 kDa β-subunits of the wild-type AGA,in addition,an aberrant polypeptide with a mol.wt of 21 kDa is also detected as a product of proteolytic cleavage of the mutant precursor molecule(PMID: 9425233).AGA can exsit as a dimer with the mol.wt between 100 kDa and 49 kDa and His124 at an interface between two heterodimers of AGA is crucial for the thermodynamically stable oligomeric structure of AGA.(PMID:9737998). It can also exsit as a heterotetrameric protein of 88 kDa(PMID:1733831).