AGAP2 Polyclonal antibody, PBS Only

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• AGAP2 Polyclonal antibody, PBS Only (14690-1-PBS)

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  WB analysis of human brain using 14690-1-AP (same clone as 14690-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 14690-1-AP (same clone as 14690-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 14690-1-AP (same clone as 14690-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 14690-1-AP (same clone as 14690-1-PBS)

  IP result of anti-AGAP2 (IP:14690-1-AP, 4ug; Detection:14690-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14690-1-AP (same clone as 14690-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14690-1-AP (AGAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14690-1-AP (same clone as 14690-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14690-1-AP (AGAP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14690-1-AP (same clone as 14690-1-PBS)

  Immunofluorescent analysis of Hela cells, using AGAP2 antibody 14690-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

AGAP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 14690-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

14690-1-PBS targets AGAP2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6142

  Product name: Recombinant human AGAP2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 848-1192 aa of BC028020

  Sequence: MVKKQRRKKLTTPSKTEGSAGQAEEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSICVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPSRDSSREERESWIRAKYEQLLFLAPLSTSEEPLGRQLWAAVQAQDVATVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSAATTPSAATTPSITATPSPRRRSSAASVGRADAPVALV

• Full Name: ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
• Calculated Molecular Weight: 125 kDa
• Observed Molecular Weight: 124 kDa
• GenBank Accession Number: BC028020
• Gene Symbol: AGAP2
• Gene ID (NCBI): 116986
• RRID: AB_2224214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99490
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.