AGER Polyclonal antibody
AGER Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 16346-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse lung tissue |
Positive IHC detected in | human renal cell carcinoma tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 11 publications below |
IHC | See 3 publications below |
IF | See 5 publications below |
Product Information
The immunogen of 16346-1-AP is AGER Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | AGER fusion protein Ag9540 |
Full Name | advanced glycosylation end product-specific receptor |
Calculated molecular weight | 404 aa, 43 kDa |
Observed molecular weight | 43 kDa |
GenBank accession number | BC020669 |
Gene symbol | AGER |
Gene ID (NCBI) | 177 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for AGER antibody 16346-1-AP | Download protocol |
IHC protocol for AGER antibody 16346-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Neurosci Lett Aqueous extracts of se-enriched Auricularia auricular attenuates D-galactose-induced cognitive deficits, oxidative stress and neuroinflammation via suppressing RAGE/MAPK/NF-κB pathway. | ||
Hum Exp Toxicol IL-12p40 and sRAGE in serum correlate with chemically induced cleft palate in mice. | ||
Environ Toxicol Pharmacol Lower concentrations of receptor for advanced glycation end products and epiregulin in amniotic fluid correlate to chemically induced cleft palate in mice. | ||
Biochem Biophys Res Commun HMGB1 mediates homocysteine-induced endothelial cells pyroptosis via cathepsin V-dependent pathway. | ||
Diabetes Res Clin Pract Heparanase-driven inflammation from the AGEs-stimulated macrophages changes the functions of glomerular endothelial cells. | ||