AGER Polyclonal antibody

AGER Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16346-1-AP

Synonyms

AGER, RAGE



Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman renal cell carcinoma tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16346-1-AP is AGER Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGER fusion protein Ag9540
Full Name advanced glycosylation end product-specific receptor
Calculated molecular weight 404 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC020669
Gene symbol AGER
Gene ID (NCBI) 177
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.

Protocols

Product Specific Protocols
WB protocol for AGER antibody 16346-1-APDownload protocol
IHC protocol for AGER antibody 16346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neurosci Lett

Aqueous extracts of se-enriched Auricularia auricular attenuates D-galactose-induced cognitive deficits, oxidative stress and neuroinflammation via suppressing RAGE/MAPK/NF-κB pathway.

Authors - Jingjing Wang
mouseIHC

Hum Exp Toxicol

IL-12p40 and sRAGE in serum correlate with chemically induced cleft palate in mice.

Authors - X-H Wang
mouseIHC

Environ Toxicol Pharmacol

Lower concentrations of receptor for advanced glycation end products and epiregulin in amniotic fluid correlate to chemically induced cleft palate in mice.

Authors - Xinhuan Wang
human,mouseWB

Biochem Biophys Res Commun

HMGB1 mediates homocysteine-induced endothelial cells pyroptosis via cathepsin V-dependent pathway.

Authors - Yiping Leng
mouseWB

Diabetes Res Clin Pract

Heparanase-driven inflammation from the AGEs-stimulated macrophages changes the functions of glomerular endothelial cells.

Authors - Guang Xu
humanWB

Neurotox Res

PMID: 31858421

Authors - Min Huang