AGK Polyclonal antibody

• Home
• Products
• Primary Antibodies
• AGK Polyclonal antibody (33759-1-AP)

• 

  WB analysis using 33759-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33759-1-AP (AGK antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 33759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 33759-1-AP (AGK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AGK Polyclonal Antibody for WB, IHC, ELISA

Cat No. 33759-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Acylglycerol kinase, mitochondrial, EC:2.7.1.94, hAGK, HsMuLK, MuLK

---

Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, K-562 cells, L02 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33759-1-AP targets AGK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag37404

  Product name: Recombinant human AGK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 93-423 aa of BC022777

  Sequence: MDVTIVKTDYEGQAKKLLELMENTDVIIVAGGDGTLQEVVTGVLRRTDEATFSKIPIGFIPLGETSSLSHTLFAESGNKVQHITDATLAIVKGETVPLDVLQIKGEKEQPVFAMTGLRWGSFRDAGVKVSKYWYLGPLKIKAAHFFSTLKEWPQTHQASISYTGPTERPPNEPEETPVQRPSLYRRILRRLASYWAQPQDALSQEVSPEVWKDVQLSTIELSITTRNNQLDPTSKEDFLNICIEPDTISKGDFITIGSRKVRNPKLHVEGTECLQASQCTLLIPEGAGGSFSIDSEEYEAMPVEVKLLPRKLQFFCDPRKREQMLTSPTQ

• Full Name: acylglycerol kinase
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC022777
• Gene Symbol: AGK
• Gene ID (NCBI): 55750
• RRID: AB_3743070
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q53H12
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGK (Acylglycerol Kinase), also designated as MULK (multi-substrate lipid kinase), is a member of the AGK protein family and functions as a lipid kinase localized to the inner mitochondrial membrane. This protein exhibits a distinctive dual functionality. First, acting as a lipid kinase, it catalyzes the phosphorylation of monoacylglycerol and diacylglycerol to yield lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively, thereby participating in phospholipid metabolism and lipid-mediated signal transduction. Second, independent of its kinase activity, AGK serves as a core subunit of the TIM22 complex, where it is responsible for the translocation and insertion of multiple mitochondrial carrier proteins (e.g., ANT1) into the inner mitochondrial membrane.