AGPAT1 Polyclonal antibody

AGPAT1 Polyclonal Antibody for IHC, IP, ELISA

Cat No. 10601-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

1 AGP acyltransferase 1, 1 AGPAT1, 1-acylglycerol-3-phosphate O-acyltransferase 1, 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha, 1-AGP acyltransferase 1

Formulation: PBS, Azide, Glycerol

Conjugate: Unconjugated

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Filter: AllIPIHC

IP experiment of Raji using 10601-1-AP
IP result of anti-AGPAT1 (IP:10601-1-AP, 4ug; Detection:10601-1-AP 1:300) with Raji cells lysate 2000ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse skeletal muscle using 10601-1-AP
IP result of anti-AGPAT1 (IP:10601-1-AP, 4ug; Detection:10601-1-AP 1:300) with mouse skeletal muscle tissue lysate 2200ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skeletal muscle using 10601-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10601-1-AP (AGPAT1 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skeletal muscle using 10601-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10601-1-AP (AGPAT1 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 10601-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10601-1-AP (AGPAT1 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 10601-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10601-1-AP (AGPAT1 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)

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MSDS SDS

Tested Applications

Positive IP detected in	Raji cells, mouse skeletal muscle tissue
Positive IHC detected in	human skeletal muscle tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10601-1-AP targets AGPAT1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0837 Product name: Recombinant human AGPAT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-283 aa of BC003007 Sequence: MDLWPGAWMLLLLLFLLLLFLLPTLWFCSPSAKYFFKMAFYNGWILFLAVLAIPVCAVRGRNVENMKILRLMLLHIKYLYGIRVEVRGAHHFPPSQPYVVVSNHQSSLDLLGMMEVLPGRCVPIAKRELLWAGSAGLACWLAGVIFIDRKRTGDAISVMSEVAQTLLTQDVRVWVFPEGTRNHNGSMLPFKRGAFHLAVQAQVPIVPIVMSSYQDFYCKKERRFTSGQCQVRVLPPVPTEGLTPDDVPALADRVRHSMLTVFREISTDGRGGGDYLKKPGGGG Predict reactive species
Full Name	1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase, alpha)
Calculated Molecular Weight	32 kDa
Observed Molecular Weight	32 kDa
GenBank Accession Number	BC003007
Gene Symbol	AGPAT1
Gene ID (NCBI)	10554
RRID	AB_2224932
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99943
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The AGPAT1 polyclonal antibody (Cat# 10601-1-AP) has 1 citation. It appears in Pharmacological Research (IF 12.2), published in 2025. The study investigated iron overload and miR-122 synergism in rescuing lipid accumulation and peroxidation in MASLD (metabolic dysfunction-associated steatotic liver disease), using the antibody for Western blot in mouse samples. The research field encompasses lipid metabolism, liver disease, and pharmacology.

Species	Application	Title
mouse	WB	Pharmacol Res Breaking the synergism of iron overload and miR-122 to rescue lipid accumulation and peroxidation in MASLD Authors - Yuxiao Tang View Article