AGPAT6 Polyclonal antibody

AGPAT6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16762-1-AP

Synonyms

1 AGP acyltransferase 6, 1 AGPAT 6, AGPAT6, GPAT4, LPAAT zeta, LPAATZ, TSARG7

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Validation Data Gallery

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  WB analysis using 16762-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16762-1-AP (AGPAT6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16762-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16762-1-AP (AGPAT6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16762-1-AP

  IP result of anti-AGPAT6 (IP:16762-1-AP, 4ug; Detection:16762-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

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  IHC staining of human testis using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse testis tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human testis tissue, human kidney tissue, human liver tissue, human ovary tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

16762-1-AP targets AGPAT6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AGPAT6 fusion protein Ag10294
• Full Name: 1-acylglycerol-3-phosphate O-acyltransferase 6 (lysophosphatidic acid acyltransferase, zeta)
• Calculated Molecular Weight: 456 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC051377
• Gene Symbol: AGPAT6
• Gene ID (NCBI): 137964
• RRID: AB_1850878
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGPAT6 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) family. AGPAT6 is expressed in brown adipose tissue and mammary epithelium as well as many other tissues. A deficiency of AGPAT6 in mice results in resistance to dietinduced and genetic forms of obesity.

Publications

Species	Application	Title
human	WB	Invest Ophthalmol Vis Sci; Alteration in Lysophospholipids and Converting Enzymes in Glaucomatous Optic Nerves.; Authors - Sasha M Milbeck
mouse	WB	EMBO Rep; MTCH2 cooperates with MFN2 and lysophosphatidic acid synthesis to sustain mitochondrial fusion; Authors - Andres Goldman