AGRP Polyclonal antibody

AGRP Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 21249-1-AP

Synonyms

Agouti related protein, AGRP, AGRT, ART, ASIP2, KNOTTIN

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Validation Data Gallery

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  IHC staining of mouse brain using 21249-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21249-1-AP (AGRP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21249-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21249-1-AP (AGRP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus using 21249-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21249-1-AP (AGRP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human oesophagus using 21249-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21249-1-AP (AGRP Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: mouse brain tissue, human oesophagus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21249-1-AP is AGRP Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AGRP fusion protein Ag15752
• Full Name: agouti related protein homolog (mouse)
• Calculated molecular weight: 132 aa, 14 kDa
• GenBank accession number: BC110443
• Gene symbol: AGRP
• Gene ID (NCBI): 181
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.