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AGXT Polyclonal antibody, PBS Only (22394-1-PBS)

AGXT Polyclonal antibody, PBS Only

Name: AGXT Polyclonal antibody, PBS Only,
Brand: Proteintech

AGXT Polyclonal Antibody for WB, ELISA

Cat No. 22394-1-PBS

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

AGT, AGT1, AGXT1, Alanine--glyoxylate aminotransferase, EC:2.6.1.44

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

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Filter: AllWBIHC

WB analysis using 22394-1-AP (same clone as 22394-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 22394-1-AP (AGXT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22394-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse lung using 22394-1-AP (same clone as 22394-1-PBS)
mouse lung tissue were subjected to SDS PAGE followed by western blot with 22394-1-AP (AGXT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22394-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 22394-1-AP
Immunohistochemical analysis of paraffin-embedded human liver slide using 22394-1-AP (AGXT Antibody) at dilution of 1:50.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 22394-1-AP
Immunohistochemical analysis of paraffin-embedded human liver slide using 22394-1-AP (AGXT Antibody) at dilution of 1:50.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications
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Product Information

22394-1-PBS targets AGXT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18062 Product name: Recombinant human AGXT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-392 aa of BC132819 Sequence: MASHKLLVTPPKALLKPLSIPNQLLLGPGPSNLPPRIMAAGGLQMIGSMSKDMYQIMDEIKEGIQYVFQTRNPLTLVISGSGHCALEAALVNVLEPGDSFLVGANGIWGQRAVDIGERIGARVHPMTKDPGGHYTLQEVEEGLAQHKPVLLFLTHGESSTGVLQPLDGFGELCHRYKCLLLVDSVASLGGTPLYMDRQGIDILYSGSQKALNAPPGTSLISFSDKAKKKMYSRKTKPFSFYLDIKWLANFWGCDDQPRMYHHTIPVISLYSLRESLALIAEQGLENSWRQHREAAAYLHGRLQALGLQLFVKDPALRLPTVTTVAVPAGYDWRDIVSYVIDHFDIEIMGGLGPSTGKVLRIGLLGCNATRENVDRVTEALRAALQHCPKKKL Predict reactive species
Full Name	alanine-glyoxylate aminotransferase
Calculated Molecular Weight	392 aa, 43 kDa
Observed Molecular Weight	43-45 kDa
GenBank Accession Number	BC132819
Gene Symbol	AGXT
Gene ID (NCBI)	189
RRID	AB_2879098
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P21549
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease that is characterized by deficient AGXT, and excessive hepatic oxalate production that leads to renal failure.