AGXT Monoclonal antibody, PBS Only

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  WB analysis using 67402-1-Ig (same clone as 67402-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67402-1-Ig (AGXT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67402-1-PBS in a different storage buffer formulation.

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  WB analysis of NCI-H1299 using 67402-1-Ig (same clone as 67402-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67402-1-Ig (AGXT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67402-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 67402-1-Ig (same clone as 67402-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67402-1-Ig (AGXT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67402-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67402-1-Ig (same clone as 67402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67402-1-Ig (AGXT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67402-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67402-1-Ig (same clone as 67402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67402-1-Ig (AGXT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67402-1-PBS in a different storage buffer formulation.

AGXT Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67402-1-PBS

Clone No.3E12H1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

3E12H1, AGT, AGT1, AGXT1, Alanine--glyoxylate aminotransferase

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Product Information

67402-1-PBS targets AGXT in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19188

  Product name: Recombinant human AGXT protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-392 aa of BC132819

  Sequence: MASHKLLVTPPKALLKPLSIPNQLLLGPGPSNLPPRIMAAGGLQMIGSMSKDMYQIMDEIKEGIQYVFQTRNPLTLVISGSGHCALEAALVNVLEPGDSFLVGANGIWGQRAVDIGERIGARVHPMTKDPGGHYTLQEVEEGLAQHKPVLLFLTHGESSTGVLQPLDGFGELCHRYKCLLLVDSVASLGGTPLYMDRQGIDILYSGSQKALNAPPGTSLISFSDKAKKKMYSRKTKPFSFYLDIKWLANFWGCDDQPRMYHHTIPVISLYSLRESLALIAEQGLENSWRQHREAAAYLHGRLQALGLQLFVKDPALRLPTVTTVAVPAGYDWRDIVSYVIDHFDIEIMGGLGPSTGKVLRIGLLGCNATRENVDRVTEALRAALQHCPKKKL

• Full Name: alanine-glyoxylate aminotransferase
• Calculated Molecular Weight: 392 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC132819
• Gene Symbol: AGXT
• Gene ID (NCBI): 189
• RRID: AB_2882644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21549
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure.