Tested Applications
| Positive WB detected in | mouse heart tissue, rat heart |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16449-1-AP targets AGXT2L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8391 Product name: Recombinant human AGXT2L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 276-450 aa of BC008009 Sequence: MGKSIGNGHPVACVAATQPVARAFEATGVEYFNTFGGSPVSCAVGLAVLNVLEKEQLQDHATSVGSFLMQLLGQQKIKHPIVGDVRGVGLFIGVDLIKDEATRTPATEEAAYLVSRLKENYVLLSTDGPGRNILKFKPPMCFSLDNARQVVAKLDAILTDMEEKVRSCETLRLQP Predict reactive species |
| Full Name | alanine-glyoxylate aminotransferase 2-like 2 |
| Calculated Molecular Weight | 450aa,50 kDa; 175aa,19 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC008009 |
| Gene Symbol | AGXT2L2 |
| Gene ID (NCBI) | 85007 |
| RRID | AB_2225868 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IUZ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AGXT2L2, also known as alanine-glyoxylate aminotransferase 2-like 2 or PHYKPL (5-phosphohydroxy-L-lysine phospho-lyase), is a nuclear gene encoding a mitochondrial enzyme. It belongs to the class-III pyridoxal phosphate-dependent aminotransferase family and shares approximately 36% sequence identity with AGXT2.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for AGXT2L2 antibody 16449-1-AP | Download protocol |
| WB protocol for AGXT2L2 antibody 16449-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AGXT2L2 Polyclonal antibody (Cat# 16449-1-AP) has 1 citation to date. It was cited in BMC Musculoskeletal Disorders (2025) in a study constructing a prognostic model based on metabolism-related gene expression in osteosarcoma patients. The research field is oncology, specifically bone tumor (osteosarcoma) prognosis and metabolic signature analysis. The antibody was used for Western blot (WB) on human samples.
| Species | Application | Title |
|---|---|---|
BMC Musculoskelet Disord Construction and evaluation of a prognostic model based on the expression of the metabolism-related signatures in patients with osteosarcoma |
