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AHCY Polyclonal antibody

AHCY Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10757-2-AP

Synonyms

Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH



Tested Applications

Positive WB detected inCOLO 320 cells, HepG2 cells, mouse liver tissue, HeLa cells, Jurkat cells, rat liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10757-2-AP targets AHCY in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Branchiostoma belcheri
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AHCY fusion protein Ag1208
Full Name S-adenosylhomocysteine hydrolase
Calculated molecular weight 48 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC011606
Gene symbol AHCY
Gene ID (NCBI) 191
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).

Protocols

Product Specific Protocols
WB protocol for AHCY antibody 10757-2-APDownload protocol
IHC protocol for AHCY antibody 10757-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Longev Healthspan

Altered dietary methionine differentially impacts glutathione and methionine metabolism in long-living growth hormone-deficient Ames dwarf and wild-type mice.

Authors - Holly M Brown-Borg
human,mouseWB

Toxicol Sci

Identification of Serum Biomarkers to Distinguish Hazardous and Benign Aminotransferase Elevations.

Authors - Joel H Vazquez
humanWB

Cell Stem Cell

Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.

Authors - Rashim Pal Singh
Branchiostoma belcheriWB,IHC

Biosci Rep

Characterization, expression and localization of S-adenosylhomocysteine hydrolase from amphioxus Branchiostoma belcheri tsingtaunese.

Authors - Wang Yuan Y
ratWB

Environ Toxicol Pharmacol

Ligustrazine modulates renal cysteine biosynthesis in rats exposed to cadmium.

Authors - Wenhua Kuang
humanWB

Mol Biol Rep

Overexpression of S-adenosylhomocysteine hydrolase (SAHH) in esophageal squamous cell carcinoma (ESCC) cell lines: effects on apoptosis, migration and adhesion of cells.

Authors - Qinghua Li