AHCYL1 Polyclonal antibody

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• AHCYL1 Polyclonal antibody (10658-3-AP)

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AHCYL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10658-3-AP Copied!

Publications (23)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA
For FC, select conjugated version.

Synonyms

AHCYL 1, DCAL, DC-expressed AHCY-like molecule, IP(3)Rs binding protein released with IP(3), IRBIT

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  WB analysis of HeLa using 10658-3-AP

  WB result of AHCYL1 antibody (10658-3-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AHCYL1 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 10658-3-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10658-3-AP (AHCYL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse thymus using 10658-3-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10658-3-AP (AHCYL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 10658-3-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10658-3-AP (AHCYL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 10658-3-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10658-3-AP (AHCYL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 10658-3-AP

  IP result of anti-AHCYL1 (IP:10658-3-AP, 4ug; Detection:10658-3-AP 1:1000) with HeLa cells lysate 2400ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 10658-3-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10658-3-AP (AHCYL1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 10658-3-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10658-3-AP (AHCYL1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 10658-3-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AHCYL1 antibody (10658-3-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, mouse thymus tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10658-3-AP targets AHCYL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1017

  Product name: Recombinant human AHCYL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 212-530 aa of BC007576

  Sequence: IDRCVNMDGWQANMILDDGGDLTHWVYKKYPNVFKKIRGIVEESVTGVHRLYQLSKAGKLCVPAMNVNDSVTKQKFDNLYCCRESILDGLKRTTDVMFGGKQVVVCGYGEVGKGCCAALKALGAIVYITEIDPICALQACMDGFRVVKLNEVIRQVDVVITCTGNKNVVTREHLDRMKNSCIVCNMGHSNTEIDVTSLRTPELTWERVRSQVDHVIWPDGKRVVLLAEGRLLNLSCSTVPTFVLSITATTQALALIELYNAPEGRYKQDVYLLPKKMDEYVASLHLPSFDAHLTELTDDQAKYLGLNKNGPFKPNYYRY

• Full Name: S-adenosylhomocysteine hydrolase-like 1
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC007576
• Gene Symbol: AHCYL1
• Gene ID (NCBI): 10768
• RRID: AB_2226013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43865
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1), also known as IP3 receptor-binding protein released with IP3 (IRBIT), regulates IP3-induced Ca2+ release into the cytoplasm of cells(PMID:23145124). This protein is also named as DCAL, XPVKONA and belongs to the adenosylhomocysteinase family. AHCYL1 mRNA and protein are most abundant in the glandular epithelium of adenocarcinoma of cancerous, but not normal and AHCYL1 protein is predominantly in the nucleus(PMID:22826361). It has 2 isoforms produced by alternative splicing.

Publications

What published studies show

The AHCYL1 (IRBIT) Polyclonal antibody (Cat# 10658-3-AP) has 23 citations. Journals include Molecular Cell, Cell Death & Differentiation, Journal of Agricultural and Food Chemistry, Molecular Nutrition & Food Research, Poultry Science, Nutrients, Journal of Gastroenterology, PLoS ONE, European Journal of Nutrition, Journal of Nutrition, Neuroscience, Pharmaceutics, Anticancer Research, and others. Research fields span epigenetics and DNA methylation, betaine supplementation, hepatic lipid/cholesterol metabolism, cancer, neurogenesis, ferroptosis, and pancreatitis, with applications mainly in WB, IHC, and IP.

Automatically generated from the 23 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Mol Cell; Thermo-sensing mechanisms of splicing control by nuclear stress bodies.; Authors - Tsuyoshi Ueno
human	WB,IP	Cell Death Differ; Quantitative reactive cysteinome profiling reveals a functional link between ferroptosis and proteasome-mediated degradation; Authors - Yankun Wang
pig	WB	J Anim Sci Biotechnol; Betaine alleviates hepatic lipid metabolism disorder in finishing pigs fed a low-energy diet through regulating m(6)A RNA methylation.; Authors - Chan Liang
human	WB	Pharmaceutics; Protective Role of IRBIT on Sodium Bicarbonate Cotransporter-n1 for Migratory Cancer Cells.; Authors - Soyoung Hwang
pig	WB	J Agric Food Chem; Down-regulation of miR-150 expression by DNA hypermethylation is associated with high HMB-induced hepatic cholesterol accumulation in nursery piglets.; Authors - Yimin Jia
pig	WB	J Agric Food Chem; Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.; Authors - Yimin Jia