Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IHC detected in | human renal cell carcinoma tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17682-1-AP targets AHNAK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11779 Product name: Recombinant human AHNAK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 134-483 aa of BC049216 Sequence: EQPVDLNLPLEAPPISKVRVHIQGAQVESQEVTIHSIVTPEFVDLSVPRTFSTQIVRESEIPTSEIQTPSYGFSLLKVKIPEPHTQARVYTTMTQHSRTQEGTEEAPIQATPGVDSISGDLQPDTGEPFEMISSSVNVLGQQTLTFEVPSGHQLADSCSDEEPAEILEFPPDDSQEATTPLADEGRAPKDKPESKKSGLLWFWLPNIGFSSSVDETGVDSKNDVQRSAPIQTQPEARPEAELPKKQEKAGWFRFPKLGFSSSPTKKSKSTEDGAELEEQKLQEETITFFDARESFSPEEKEEGELIGPVGTGLDSRVMVTSAARTELILPEQDRKADDESKGSGLGPNEG Predict reactive species |
| Full Name | AHNAK nucleoprotein 2 |
| Calculated Molecular Weight | 617 kDa |
| Observed Molecular Weight | 617 kDa |
| GenBank Accession Number | BC049216 |
| Gene Symbol | AHNAK2 |
| Gene ID (NCBI) | 113146 |
| RRID | AB_2878428 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IVF2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AHNAK2 antibody 17682-1-AP | Download protocol |
| IHC protocol for AHNAK2 antibody 17682-1-AP | Download protocol |
| WB protocol for AHNAK2 antibody 17682-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AHNAK2 antibody (Cat# 17682-1-AP) has 11 citations published in journals including Cell Reports Medicine, International Immunopharmacology, Clinical and Experimental Immunology, Frontiers in Oncology, Journal of Gene Medicine, Discover Oncology, FEBS Open Bio, Cancer Management and Research, Onco Targets and Therapy, and Heliyon. Research fields span oncology (thyroid, lung, gastric, pancreatic, cervical, and chordoma cancers), pulmonary fibrosis, immunology, and reproductive medicine, with applications in WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Cell Rep Med Clinical-proteomic classification and precision treatment strategy of chordoma | ||
Int Immunopharmacol AHNAK2 is a novel prognostic marker and correlates with immune infiltration in papillary thyroid cancer: Evidence from integrated analysis. | ||
Int Immunopharmacol Correlation between prognostic indicator AHNAK2 and immune infiltrates in lung adenocarcinoma. | ||
Clin Exp Immunol The AHNAK induces increased IL-6 production in CD4+T cells and serves as a potential diagnostic biomarker for recurrent pregnancy loss. | ||
Front Oncol The genetic landscape of primary malignant melanoma of the cervix using integrated bioinformatics analysis and whole-exome sequencing. | ||
J Gene Med Inhibition of AHNAK nucleoprotein 2 alleviates pulmonary fibrosis by downregulating the TGF-β1/Smad3 signaling pathway. |
