AHNAK2 Polyclonal antibody

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• AHNAK2 Polyclonal antibody (17682-1-AP)

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• KD/KO Validated

AHNAK2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17682-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AHNAK nucleoprotein 2, C14orf78, Protein AHNAK2

• 

  WB analysis using 17682-1-AP

  Various lysates were subjected to Tris-acetate gel system followed by western blot with 17682-1-AP (AHNAK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human renal cell carcinoma using 17682-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 17682-1-AP (AHNAK2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human renal cell carcinoma using 17682-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 17682-1-AP (AHNAK2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 17682-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17682-1-AP (AHNAK2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 17682-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17682-1-AP (AHNAK2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U2OS using 17682-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 17682-1-AP (AHNAK2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human renal cell carcinoma tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17682-1-AP targets AHNAK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11779

  Product name: Recombinant human AHNAK2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 134-483 aa of BC049216

  Sequence: EQPVDLNLPLEAPPISKVRVHIQGAQVESQEVTIHSIVTPEFVDLSVPRTFSTQIVRESEIPTSEIQTPSYGFSLLKVKIPEPHTQARVYTTMTQHSRTQEGTEEAPIQATPGVDSISGDLQPDTGEPFEMISSSVNVLGQQTLTFEVPSGHQLADSCSDEEPAEILEFPPDDSQEATTPLADEGRAPKDKPESKKSGLLWFWLPNIGFSSSVDETGVDSKNDVQRSAPIQTQPEARPEAELPKKQEKAGWFRFPKLGFSSSPTKKSKSTEDGAELEEQKLQEETITFFDARESFSPEEKEEGELIGPVGTGLDSRVMVTSAARTELILPEQDRKADDESKGSGLGPNEG

• Full Name: AHNAK nucleoprotein 2
• Calculated Molecular Weight: 617 kDa
• Observed Molecular Weight: 617 kDa
• GenBank Accession Number: BC049216
• Gene Symbol: AHNAK2
• Gene ID (NCBI): 113146
• RRID: AB_2878428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IVF2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Int Immunopharmacol; Correlation between prognostic indicator AHNAK2 and immune infiltrates in lung adenocarcinoma.; Authors - Miaosen Zheng
human	IHC	Int Immunopharmacol; AHNAK2 is a novel prognostic marker and correlates with immune infiltration in papillary thyroid cancer: Evidence from integrated analysis.; Authors - Long Zheng
human	IHC,IF	Clin Exp Immunol; The AHNAK induces increased IL-6 production in CD4+T cells and serves as a potential diagnostic biomarker for recurrent pregnancy loss.; Authors - Liman Li
human	WB	Onco Targets Ther; AHNAK2 Promotes Migration, Invasion, and Epithelial-Mesenchymal Transition in Lung Adenocarcinoma Cells via the TGF-β/Smad3 Pathway.; Authors - Gang Liu ; KD Validated
mouse,human	WB,IHC,IF	Cancer Manag Res; AHNAK2 Promotes the Progression of Pancreatic Ductal Adenocarcinoma by Maintaining the Stability of c-MET; Authors - Zhaohui Chen
human	IHC	FEBS Open Bio; Methylation of drug resistance-related genes in chemotherapy-sensitive Epstein-Barr virus-associated gastric cancer.; Authors - Hirofumi Ohmura