AHR Recombinant monoclonal antibody, PBS Only

• 

  IHC staining of mouse testis using 83369-6-RR (same clone as 83369-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83369-6-RR (AHR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83369-6-RR (same clone as 83369-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83369-6-RR (same clone as 83369-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83369-6-RR (same clone as 83369-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83369-6-RR (same clone as 83369-6-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug AHR Recombinant antibody (83369-6-RR, Clone:240325G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 83369-6-RR (same clone as 83369-6-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug AHR Recombinant antibody (83369-6-RR, Clone:240325G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

AHR Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Cat No. 83369-6-PBS

Clone No.240325G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor, 240325G9

---

Product Information

83369-6-PBS targets AHR in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag12193

  Product name: Recombinant human AHR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-387 aa of BC070080

  Sequence: MNSSSANITYASRKRRKPVQKTVKPIPAEGIKSNPSKRHRDRLNTELDRLASLLPFPQDVINKLDKLSVLRLSVSYLRAKSFFDVALKSSPTERNGGQDNCRAANFREGLNLQEGEFLLQALNGFVLVVTTDALVFYASSTIQDYLGFQQSDVIHQSVYELIHTEDRAEFQRQLHWALNPSQCTESGQGIEEATGLPQTVVCYNPDQIPPENSPLMERCFICRLRCLLDNSSGFLAMNFQGKLKYLHGQKKKGKDGSILPPQLALFAIATPLQPPSILEIRTKNFIFRTKHKLDFTPIGCDAKGRIVLGYTEAELCTRGSGYQFIHAADMLYCAESHIRMIKTGESGMIVFRLLTKNNRWTWVQSNARLLYKNGRPDYIIVTQRPLT

• Full Name: aryl hydrocarbon receptor
• Calculated Molecular Weight: 848 aa, 96 kDa
• GenBank Accession Number: BC070080
• Gene Symbol: AHR
• Gene ID (NCBI): 196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P35869
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism (PMID:18483242). It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH) 17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling (PMID:20423157,18540824). It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)