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  • KD/KO Validated

AHSA1 Polyclonal antibody

AHSA1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14725-1-AP

Synonyms

AHA1, AHSA1, C14orf3



Tested Applications

Positive WB detected inA431 cells, HeLa cells, mouse brain tissue, HEK-293 cells, A549 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14725-1-AP targets AHSA1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AHSA1 fusion protein Ag6482
Full Name AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
Calculated molecular weight 338 aa, 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000321
Gene symbol AHSA1
Gene ID (NCBI) 10598
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AHSA1 antibody 14725-1-APDownload protocol
IHC protocol for AHSA1 antibody 14725-1-APDownload protocol
IF protocol for AHSA1 antibody 14725-1-APDownload protocol
IP protocol for AHSA1 antibody 14725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Front Immunol

The Prognostic and Immunotherapeutic Significance of AHSA1 in Pan-Cancer, and Its Relationship With the Proliferation and Metastasis of Hepatocellular Carcinoma.

Authors - Wenli Li
  • KO Validated
humanWB

Cancers (Basel)

AHSA1 Promotes Proliferation and EMT by Regulating ERK/CALD1 Axis in Hepatocellular Carcinoma

Authors - Jiakang Zhang
  • KD Validated