Various lysates were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14725-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14725-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 14725-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
A431 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14725-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 14725-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
A549 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 14725-1-AP
IP result of anti-AHSA1 (IP:14725-1-AP, 4ug; Detection:14725-1-AP 1:5000) with mouse brain tissue lysate 1280 ug.
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14725-1-AP (AHSA1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 14725-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using AHSA1 antibody (14725-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using AHSA1 antibody (14725-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
A431 cells, HeLa cells, mouse brain tissue, HEK-293 cells, A549 cells, rat brain tissue
Positive IP detected in
mouse brain tissue
Positive IHC detected in
human gliomas tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
A431 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:3000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
The Prognostic and Immunotherapeutic Significance of AHSA1 in Pan-Cancer, and Its Relationship With the Proliferation and Metastasis of Hepatocellular Carcinoma.
Various lysates were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14725-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14725-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 14725-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14725-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 14725-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human gliomas using 14725-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 14725-1-AP (AHSA1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human gliomas using 14725-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 14725-1-AP (AHSA1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse skeletal muscle using 14725-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14725-1-AP (AHSA1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of mouse brain using 14725-1-AP
IP result of anti-AHSA1 (IP:14725-1-AP, 4ug; Detection:14725-1-AP 1:5000) with mouse brain tissue lysate 1280 ug.
IF/ICC Figures
IF Staining of A431 using 14725-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using AHSA1 antibody (14725-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
AHSA1 Polyclonal antibody
Catalog Number
14725-1-AP
Citations
3
Dilutions
WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
