AHSA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

AHSA1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14725-1-AP

Synonyms

AHA1, AHSA1, C14orf3

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Validation Data Gallery

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  WB analysis using 14725-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14725-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14725-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 14725-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14725-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14725-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14725-1-AP

  IP result of anti-AHSA1(IP:14725-1-AP, 4ug; Detection:14725-1-AP 1:5000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of human gliomas using 14725-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14725-1-AP (AHSA1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 14725-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14725-1-AP (AHSA1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse skeletal muscle using 14725-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14725-1-AP (AHSA1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 14725-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using AHSA1 antibody (14725-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, mouse brain tissue, HEK-293 cells, A549 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

14725-1-AP targets AHSA1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AHSA1 fusion protein Ag6482
• Full Name: AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
• Calculated molecular weight: 338 aa, 38 kDa
• Observed molecular weight: 38 kDa
• GenBank accession number: BC000321
• Gene symbol: AHSA1
• Gene ID (NCBI): 10598
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Front Immunol; The Prognostic and Immunotherapeutic Significance of AHSA1 in Pan-Cancer, and Its Relationship With the Proliferation and Metastasis of Hepatocellular Carcinoma.; Authors - Wenli Li ; KO Validated
human	WB	Cancers (Basel); AHSA1 Promotes Proliferation and EMT by Regulating ERK/CALD1 Axis in Hepatocellular Carcinoma; Authors - Jiakang Zhang ; KD Validated