AIF Recombinant antibody

AIF Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 86156-3-RR
Clone No.250772F8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

AIFM1, Apoptosis-inducing factor 1, mitochondrial, EC:1.6.99.-, PDCD8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, Jurkat cells, K-562 cells, NIH/3T3 cells, C2C12 cells, C6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86156-3-RR targets AIF in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AIF fusion protein Ag13268 Predict reactive species
Full Name apoptosis-inducing factor, mitochondrion-associated, 1
Calculated Molecular Weight 609 aa, 66 kDa
Observed Molecular Weight67 kDa
GenBank Accession NumberBC111065
Gene Symbol AIF
Gene ID (NCBI) 9131
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95831
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).

Protocols

Product Specific Protocols
WB protocol for AIF antibody 86156-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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