AIPL1 Polyclonal antibody

AIPL1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15108-1-AP

Synonyms

AIPL1, AIPL2, LCA4

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Validation Data Gallery

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  WB analysis of Y79 using 15108-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 15108-1-AP (AIPL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15108-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15108-1-AP (AIPL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 15108-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15108-1-AP (AIPL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Y79 using 15108-1-AP

  IP result of anti-AIPL1 (IP:15108-1-AP, 4ug; Detection:15108-1-AP 1:500) with Y79 cells lysate 2400 ug.

• 

  IHC staining of human eye using 15108-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 15108-1-AP (AIPL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human eye using 15108-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 15108-1-AP (AIPL1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Y79 cells, mouse brain tissue, SH-SY5Y cells
• Positive IP detected in: Y79 cells
• Positive IHC detected in: human eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

15108-1-AP targets AIPL1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AIPL1 fusion protein Ag4625
• Full Name: aryl hydrocarbon receptor interacting protein-like 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC012055
• Gene Symbol: AIPL1
• Gene ID (NCBI): 23746
• RRID: AB_2224995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Sci Rep; ZP3 is Required for Germinal Vesicle Breakdown in Mouse Oocyte Meiosis.; Authors - Lei-Lei Gao
mouse	IP	Zygote; ZP3 and AIPL1 participate in GVBD of mouse oocytes by affecting the nuclear membrane localization and maturation of farnesylated prelamin A; Authors - Zhen Jin