AIPL1 Polyclonal antibody

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• AIPL1 Polyclonal antibody (15108-1-AP)

AIPL1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 15108-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Synonyms

AIPL1, AIPL2, LCA4

• 

  WB analysis of Y79 using 15108-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 15108-1-AP (AIPL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of SH-SY5Y using 15108-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15108-1-AP (AIPL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 15108-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15108-1-AP (AIPL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of Y79 using 15108-1-AP

  IP result of anti-AIPL1 (IP:15108-1-AP, 4ug; Detection:15108-1-AP 1:500) with Y79 cells lysate 2400 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human eye using 15108-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 15108-1-AP (AIPL1 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human eye using 15108-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 15108-1-AP (AIPL1 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Y79 cells, mouse brain tissue, SH-SY5Y cells
• Positive IP detected in: Y79 cells
• Positive IHC detected in: human eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15108-1-AP targets AIPL1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4625

  Product name: Recombinant human AIPL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 30-384 aa of BC012055

  Sequence: GSRVIFHFRTMKCDEERTVIDDSRQVGQPMHIIIGNMFKLEVWEILLTSMRVHEVAEFWCDTIHTGVYPILSRSLRQMAQGKDPTEWHVHTCGLANMFAYHTLGYEDLDELQKEPQPLVFVIELLQVDAPSDYQRETWNLSNHEKMKAVPVLHGEGNRLFKLGRYEEASSKYQEAIICLRNLQTKEKPWEVQWLKLEKMINTLILNYCQCLLKKEEYYEVLEHTSDILRHHPGIVKAYYVRARAHAEVWNEAEAKADLQKVLELEPSMQKAVRRELRLLENRMAEKQEEERLRCRNMLSQGATQPPAEPPTEPPAQSSTEPPAEPPTAPSAELSAGPPAEPATEPPPSPGHSLQH

• Full Name: aryl hydrocarbon receptor interacting protein-like 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC012055
• Gene Symbol: AIPL1
• Gene ID (NCBI): 23746
• RRID: AB_2224995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NZN9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-ZP3 antibody (catalog #15108-1-AP) has 2 total citations, appearing in Scientific Reports (IF 4.9) and Zygote (IF 1.7). Both studies focus on mouse oocyte biology, specifically germinal vesicle breakdown (GVBD) during meiosis. One study used WB and IF to demonstrate ZP3's role in GVBD, while the other employed IP to investigate ZP3's interaction with AIPL1 in nuclear membrane localization and prelamin A maturation.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	Sci Rep; ZP3 is Required for Germinal Vesicle Breakdown in Mouse Oocyte Meiosis.; Authors - Lei-Lei Gao
mouse	IP	Zygote; ZP3 and AIPL1 participate in GVBD of mouse oocytes by affecting the nuclear membrane localization and maturation of farnesylated prelamin A; Authors - Zhen Jin