AK1 Polyclonal antibody

AK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 14978-1-AP

Synonyms

adenylate kinase 1, Adenylate kinase isoenzyme 1, AK 1, AK1, ATP AMP transphosphorylase 1, Myokinase

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Validation Data Gallery

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  WB analysis using 14978-1-AP

  mouse heart lysates were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14978-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14978-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14978-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14978-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 14978-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14978-1-AP (AK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 14978-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14978-1-AP (AK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

14978-1-AP targets AK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AK1 fusion protein Ag6890
• Full Name: adenylate kinase 1
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC001116
• Gene Symbol: AK1
• Gene ID (NCBI): 203
• RRID: AB_2225148
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	J Proteome Res; Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts; Authors - Bridgette Hartley
mouse	WB	Eur J Pharmacol; Adenosine kinase (ADK) inhibition with ABT-702 induces ADK protein degradation and a distinct form of sustained cardioprotection.; Authors - Gerald Wölkart
	WB	PLoS One; Adenylate kinase 1 overexpression increases locomotor activity in medaka fish; Authors - Michiyo Maruyama
	WB	Cell Res; Hierarchical activation of compartmentalized pools of AMPK depends on severity of nutrient or energy stress.; Authors - Yue Zong
human,mouse	WB	EMBO J; Aberrant DNA N6 -methyladenine incorporation via adenylate kinase 1 is suppressed by ADAL deaminase-dependent 2'-deoxynucleotide pool sanitation; Authors - Shaokun Chen