AK5 Polyclonal antibody, PBS Only

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• AK5 Polyclonal antibody, PBS Only (12510-2-PBS)

AK5 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 12510-2-PBS Copied!

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

• 

  WB analysis using 12510-2-AP (same clone as 12510-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12510-2-AP (AK5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12510-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse cerebellum using 12510-2-AP (same clone as 12510-2-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 12510-2-AP (AK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12510-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 12510-2-AP (same clone as 12510-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12510-2-AP (AK5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12510-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 12510-2-AP (same clone as 12510-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12510-2-AP (AK5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12510-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 12510-2-AP (same clone as 12510-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12510-2-AP (AK5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12510-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 12510-2-AP (same clone as 12510-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12510-2-AP (AK5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12510-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 12510-2-AP (same clone as 12510-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12510-2-AP (AK5 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12510-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 12510-2-AP (same clone as 12510-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12510-2-AP (AK5 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12510-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

12510-2-PBS targets AK5 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3203

  Product name: Recombinant human AK5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 3-328 aa of BC012467

  Sequence: PDLVVFLACANQRLKERLLKRAEQQGRPDDNVKATQRRLMNFKQNAAPLVKYFQEKGLIMTFDADRDEDEVFYDISMAVDNKLFPNKEAAAGSSDLDPSMILDTGEIIDTGSDYEDQGDDQLNVFGEDTMGGFMEDLRKCKIIFIIGGPGSGKGTQCEKLVEKYGFTHLSTGELLREELASESERSKLIRDIMERGDLVPSGIVLELLKEAMVASLGDTRGFLIDGYPREVKQGEEFGRRIGDPQLVICMDCSADTMTNRLLQRSRSSLPVDDTTKTIAKRLEAYYRASIPVIAYYETKTQLHKINAEGTPEDVFLQLCTAIDSIF

• Full Name: adenylate kinase 5
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC012467
• Gene Symbol: AK5
• Gene ID (NCBI): 26289
• RRID: AB_2305376
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y6K8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.