Tested Applications
| Positive WB detected in | rat brain tissue |
| Positive IHC detected in | mouse brain tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25199-1-AP targets AKAP12 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17769 Product name: Recombinant human AKAP12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1335-1684 aa of BC157067 Sequence: ETANILETGETLEPAGAHLVLEEKSSEKNEDFAAHPGEDAVPTGPDCQAKSTPVIVSATTKKGLSSDLEGEKTTSLKWKSDEVDEQVACQEVKVSVAIEDLEPENGILELETKSSKLVQNIIQTAVDQFVRTEETATEMLTSELQTQAHVIKADSQDAGQETEKEGEEPQASAQDETPITSAKEESESTAVGQAHSDISKDMSEASEKTMTVEVEGSTVNDQQLEEVVLPSEEEGGGAGTKSVPEDDGHALLAERIEKSLVEPKEDEKGDDVDDPENQNSALADTDASGGLTKESPDTNGPKQKEKEDAQEVELQEGKVHSESDKAITPQAQEELQKQERESAKSELTES Predict reactive species |
| Full Name | A kinase (PRKA) anchor protein 12 |
| Calculated Molecular Weight | 1782 aa, 191 kDa |
| Observed Molecular Weight | 200-300 kDa |
| GenBank Accession Number | BC157067 |
| Gene Symbol | AKAP12 |
| Gene ID (NCBI) | 9590 |
| RRID | AB_2879954 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02952 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AKAP12, also named as AKAP250, is a 1782 amino acid protein, which contains three AKAP domains. AKAP12 localizes in the cytoplasm and is expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells. AKAP12 as an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC). AKAP12 expression is known to be suppressed in several human malignancies including breast, prostate, gastric and colon cancers. AKAP12 has three isoforms, namely AKAP12A, AKAP12B and AKAP12C, with molecular weights of 305 kDa, 287 kDa and 250 kDa respectively. (PMID: 30385176, PMID: 15258566)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for AKAP12 antibody 25199-1-AP | Download protocol |
| WB protocol for AKAP12 antibody 25199-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AKAP12 antibody (Cat# 25199-1-AP) has 15 citations spanning journals including Nature, Signal Transduction and Targeted Therapy, Theranostics, International Journal of Biological Sciences, Journal of Translational Medicine, Molecular Therapy Nucleic Acids, Respiratory Research, Frontiers in Endocrinology, Journal of Molecular Medicine, Molecular Medicine Reports, Scientific Reports, iScience, Surgery, Experimental Animals, and American Journal of Translational Research. Research fields cover liver disease, renal injury, colorectal and ovarian cancers, hepatocellular carcinoma, sepsis, endometrial carcinoma, spinal cord injury, NSCLC, kidney development, rheumatoid arthritis, and gastric cancer.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease | ||
Theranostics WT1+ glomerular parietal epithelial progenitors promote renal proximal tubule regeneration after severe acute kidney injury | ||
Int J Biol Sci CD109 Drives Stemness and Chemoresistance in Colorectal Cancer via LRRC8A/AKAP12/PKCα-Mediated STAT3 Activation and WNT Signaling. | ||
J Transl Med Prognostic risk factors of serous ovarian carcinoma based on mesenchymal stem cell phenotype and guidance for therapeutic efficacy | ||
Mol Ther Nucleic Acids The Human Novel Gene LNC-HC Inhibits Hepatocellular Carcinoma Cell Proliferation by Sequestering hsa-miR-183-5p. |
Reviews
What customers say
Users report consistent performance across applications. For immunofluorescence, the antibody works well at 1:100–1:300 dilution in mouse tissues, clearly marking structures such as Bowman's capsule in embryonic kidney. For western blotting, it reliably detects endogenous AKAP12 at 1:1000 in HEK293 and COS7 cell lines. Overall ratings are high (4–5 out of 5), with reviewers describing the antibody as "working great" and "very well" for both IF and WB. All reviews used the same lot, indicating batch consistency.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Joo-Seop (Verified Customer) (08-27-2025) | Akap12 marks Bowman's capsule in mouse embryonic kidney at E18.5.
 |
Pradeep (Verified Customer) (07-20-2020) | I used for immunofluorescence in mouse tissue sample. it is working great.
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Rushita (Verified Customer) (11-26-2019) | The Ab works very well for detection of endogenous AKAP12 for western blotting (1:1000) in HEK 293 and COS7 cell lines.
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