AKAP3 Monoclonal antibody, PBS Only

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  WB analysis of human testis using 66255-1-Ig (same clone as 66255-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66255-1-Ig (AKAP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66255-1-Ig (AKAP3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66255-1-Ig (AKAP3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (66255-1-Ig, Clone: 3C11H9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (66255-1-Ig, Clone: 3C11H9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

AKAP3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 66255-1-PBS

Clone No.3C11H9

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

A kinase anchor protein 3, AKAP 110, AKAP 3, AKAP110, AKAP3, Cancer/testis antigen 82, CT82, Fibrousheathin 1, Fibrousheathin I, FSP95, PRKA3, SOB1, Sperm oocyte binding protein

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Product Information

66255-1-PBS targets AKAP3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4885

  Product name: Recombinant human AKAP3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-353 aa of BC047535

  Sequence: MSEKVDWLQSQNGVCKVDVYSPGDNQAQDWKMDTSTDPVRVLSWLRRDLEKSTAEFQDVRFKPGESFGGETSNSGDPHKGFSVDYYNTTTKGTPERLHFEMTHKEIPCQGPRAQLGNESSVDEVSFYANRLTNLVIAMARKEINEKIDGSENKCVYQSLYMGNEPTPTKSLSKIASELVNETVSACSRNAAPDKAPGSGDRVSGSSQSPPNLKYKSTLKIKESTKERQGPDDKPPSKKSFFYKEVFESRNGDYAREGGRFFPRERKRFRGQERPDDFTASVSEGIMTYANSVVSDMMVSIMKTLKIQVKDTTIATILLKKVLLKHAKEVVSDLIDSFLRNLHSVTGTLMTDTQ

• Full Name: A kinase (PRKA) anchor protein 3
• Calculated Molecular Weight: 95 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC047535
• Gene Symbol: AKAP3
• Gene ID (NCBI): 10566
• RRID: AB_2881643
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75969
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.