AKD2 Polyclonal antibody

AKD2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27554-1-AP

Synonyms

Adenylate kinase 9, AK 9, AK9, AKD1, AKD2, C6orf199, C6orf224, RP1 70A9.1



Tested Applications

Positive WB detected inSMMC-7721 cells, HepG2 cells, HuH-7 cells
Positive IHC detected inhuman heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27554-1-AP targets AKD2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKD2 fusion protein Ag25805
Full Name adenylate kinase domain containing 2
Calculated Molecular Weight221 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC022031
Gene Symbol AKD2
Gene ID (NCBI) 221264
RRIDAB_2880904
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AK9, also named as adenylate kinase 9, belongs to adenylate kinase family, which is a small, ususlly monomeric, enzyme found in every living thing due to its crucial role in energenic metabolism(PMID: 30666486). AK9 is a nucleoside mono- and diphosphate kinase due to its broad phosphatase activity, the local concentration of substrates probably influences catalytic activity(PMID: 24495878). This antibody can detect 45-50 kDa band of AK9(PMID: 23416111).

Protocols

Product Specific Protocols
WB protocol for AKD2 antibody 27554-1-APDownload protocol
IHC protocol for AKD2 antibody 27554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Carcinog

Integrative analyses of N6-methyladenosine-associated single-nucleotide polymorphisms (m6A-SNPs) identify tumor suppressor gene AK9 in lung cancer

Authors - Tingting Hua