AKD2 Polyclonal antibody

AKD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27554-1-AP

Synonyms

Adenylate kinase 9, AK 9, AK9, AKD1, AKD2, C6orf199, C6orf224, RP1 70A9.1

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Validation Data Gallery

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  WB analysis using 27554-1-AP

  Various lysates lysate were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 27554-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 27554-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27554-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 27554-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 27554-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 27554-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27554-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SMMC-7721 cells, HepG2 cells, HuH-7 cells
• Positive IHC detected in: human heart tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

27554-1-AP targets AKD2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AKD2 fusion protein Ag25805
• Full Name: adenylate kinase domain containing 2
• Calculated Molecular Weight: 221 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC022031
• Gene Symbol: AKD2
• Gene ID (NCBI): 221264
• RRID: AB_2880904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AK9, also named as adenylate kinase 9, belongs to adenylate kinase family, which is a small, ususlly monomeric, enzyme found in every living thing due to its crucial role in energenic metabolism(PMID: 30666486). AK9 is a nucleoside mono- and diphosphate kinase due to its broad phosphatase activity, the local concentration of substrates probably influences catalytic activity(PMID: 24495878). This antibody can detect 45-50 kDa band of AK9(PMID: 23416111).

Publications

Species	Application	Title
human	WB	Mol Carcinog; Integrative analyses of N6-methyladenosine-associated single-nucleotide polymorphisms (m6A-SNPs) identify tumor suppressor gene AK9 in lung cancer; Authors - Tingting Hua