Tested Applications
| Positive IP detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22649-1-AP targets AKIRIN1 in IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18530 Product name: Recombinant human AKIRIN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-129 aa of BC119745 Sequence: MACGATLKRPMEFEAALLSPGSPKRRRCAPLPGPTPGLRPPDAEPPPPFQTQTPPQSLQQPAPPGSERRLPTPEQIFQNIKQEYSRYQRWRHLEVVLNQSEACASESQPHSSALTAPSSPGSSWMKKDQ Predict reactive species |
| Full Name | akirin 1 |
| Calculated Molecular Weight | 192 aa, 22 kDa |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | BC119745 |
| Gene Symbol | AKIRIN1 |
| Gene ID (NCBI) | 79647 |
| RRID | AB_3742075 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H9L7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Akirin1 is a highly conserved ubiquitously expressed nuclear protein harboring a clear nuclear localization signal and the protein-protein interaction property. Acting as a cofactor, Akirin1 has been disclosed that regulate transcriptional activities by establishing a link between multiple transcription factors and chromatin remodeling complexes (PMID: 39188701) . Akirin1 has been shown to be involved in the induction of cardiac hypertrophy via miR-1 and serum response factor (SRF) in mice (PMID: 30746755).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for AKIRIN1 antibody 22649-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
