Tested Applications
| Positive WB detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23785-1-AP targets AKNA in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20662 Product name: Recombinant human AKNA protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1090-1439 aa of BC055285 Sequence: RLEDSLHQPLQGSPTRPASAFDRPARTRGRPADSPATWGSHYGSKSTERLPGEPRGEEQIVPPGRQRARSSSVPREVLRLSLSSESELPSLPLFSEKSKTTKDSPQAARDGKRGVGSAGWPDRVTFRGQYTGHEYHVLSPKAVPKGNGTVSCPHCRPIRTQDAGGAVTGDPLGPPPADTLQCPLCGQVGSPPEADGPGSATSGAEKATTRRKAPSTPSPKQRSKQAGSSPRPPPGLWYLATAPPAPAPPAFAYISSVPIMPYPPAAVYYAPAGPTSAQPAAKWPPTASPPPARRHRHSIQLDLGDLEELNKALSRAVQAAESVRSTTRQMRSSLSADLRQAHSLRGSCLF Predict reactive species |
| Full Name | AT-hook transcription factor |
| Calculated Molecular Weight | 155 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC055285 |
| Gene Symbol | AKNA |
| Gene ID (NCBI) | 80709 |
| RRID | AB_2879324 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q7Z591 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AKNA also named as AT hook transcription factor is a 1439 amino-acids protein. AKNA, encodes a lymphoid-specific AT-hook transcription factor that is highly expressed by B and T lymphocytes. It coordinately regulates expression of the CD40 receptor and its ligand (CD154), two molecules that are critical for antigen-dependent B-cell development. AKNA expresses at least nine distinct transcripts, some of which are expressed in a tissue-specific manner. These transcripts are products of alternative splicing, alternative promoter usage, and alternative polyadenylation. The alternative AKNA transcripts are predicted to encode overlapping protein isoforms, some of which (p70 and p100) (PMID:15869410)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AKNA antibody 23785-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AKNA Polyclonal antibody (catalog number 23785-1-AP) has 3 total citations. These appear in three journals: Science Advances, Biomedical Research International, and Frontiers in Immunology. The associated research fields span cancer immunotherapy and neoantigen discovery, gastric cancer prognosis and EMT-related tumor suppression, and endometrial cancer progression involving STING activation and macrophage reprogramming. Overall, the citations focus on AKNA's role as a prognostic biomarker and tumor suppressor in human cancers.
| Species | Application | Title |
|---|---|---|
Sci Adv Immunopeptidomics-guided discovery and characterization of neoantigens for personalized cancer immunotherapy. | ||
Biomed Res Int AKNA Is a Potential Prognostic Biomarker in Gastric Cancer and Function as a Tumor Suppressor by Modulating EMT-Related Pathways. | ||
Front Immunol AKNA serves as a prognostic biomarker and restrains endometrial cancer progression via STING activation and macrophage reprogramming. |
