AKR1A1 Polyclonal antibody

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  WB analysis using 15054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15054-1-AP

  IP result of anti-AKR1A1 (IP:15054-1-AP, 4ug; Detection:15054-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human thyroid cancer using 15054-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15054-1-AP (AKR1A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 15054-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15054-1-AP (AKR1A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15054-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15054-1-AP (AKR1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

AKR1A1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15054-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

AKR1A 1, Alcohol dehydrogenase [NADP(+)], Aldo-keto reductase family 1 member A1, ALDR1, ALR

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Tested Applications

• Positive WB detected in: HeLa cells, L02 cells, mouse lung tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

15054-1-AP targets AKR1A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7037

  Product name: Recombinant human AKR1A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-325 aa of BC000670

  Sequence: MAASCVLLHTGQKMPLIGLGTWKSEPGQVKAAVKYALSVGYRHIDCAAIYGNEPEIGEALKEDVGPGKAVPREELFVTSKLWNTKHHPEDVEPALRKTLADLQLEYLDLYLMHWPYAFERGDNPFPKNADGTICYDSTHYKETWKALEALVAKGLVQALGLSNFNSRQIDDILSVASVRPAVLQVECHPYLAQNELIAHCQARGLEVTAYSPLGSSDRAWRDPDEPVLLEEPVVLALAEKYGRSPAQILLRWQVQRKVICIPKSITPSRILQNIKVFDFTFSPEEMKQLNALNKNWRYIVPMLTVDGKRVPRDAGHPLYPFNDPY

• Full Name: aldo-keto reductase family 1, member A1 (aldehyde reductase)
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC000670
• Gene Symbol: AKR1A1
• Gene ID (NCBI): 10327
• RRID: AB_2224138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P14550
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IP,IHC	Nature; Metabolic reprogramming by the S-nitroso-CoA reductase system protects against kidney injury.; Authors - Hua-Lin Zhou ; KO Validated
mouse	WB	J Med Chem; Identification of a Selective SCoR2 Inhibitor That Protects Against Acute Kidney Injury; Authors - Hua-Lin Zhou
mouse	WB	J Biol Chem; AKR1A1 is a novel mammalian S-nitroso-glutathione reductase.; Authors - Colin T Stomberski ; KO Validated