AKR1A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

AKR1A1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15054-1-AP

Synonyms

AKR1A1, Alcohol dehydrogenase [NADP+], Aldehyde reductase, ALDR1, ALR, ARM, DD3

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Validation Data Gallery

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  WB analysis of HeLa using 15054-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15054-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 15054-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15054-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15054-1-AP

  IP result of anti-AKR1A1 (IP:15054-1-AP, 4ug; Detection:15054-1-AP 1:500) with HeLa cells lysate 3200ug.

• 

  IF Staining of HeLa using 15054-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15054-1-AP (AKR1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human liver tissue, L02 cells, mouse lung tissue
• Positive IP detected in: HeLa cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

15054-1-AP targets AKR1A1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AKR1A1 fusion protein Ag7037
• Full Name: aldo-keto reductase family 1, member A1 (aldehyde reductase)
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC000670
• Gene Symbol: AKR1A1
• Gene ID (NCBI): 10327
• RRID: AB_2224138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IHC,IP	Nature; Metabolic reprogramming by the S-nitroso-CoA reductase system protects against kidney injury.; Authors - Hua-Lin Zhou ; KO Validated
mouse	WB	J Med Chem; Identification of a Selective SCoR2 Inhibitor That Protects Against Acute Kidney Injury; Authors - Hua-Lin Zhou
mouse	WB	J Biol Chem; AKR1A1 is a novel mammalian S-nitroso-glutathione reductase.; Authors - Colin T Stomberski ; KO Validated